Recently there has been increased interest in high nitrogen content “green” energetic materials which are designed to produce relatively non-toxic decomposition products as part of a push to decrease the environmental impact of energetic materials through all phases of their existence. With the use of a T-Jump/Time-of-Flight Mass Spectrometer (T-Jump/TOFMS) capable of high heating rates (up to ...

The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. The fluorescence intensity of BEBO increases upon binding to double-stranded DNA allowing emission to be measured at the end of the elongation phase in the PCR cycle. BE...

The first intermediates in the metabolism of thiamine by a microorganism isolated from soil are Z-methyl-4-amino-5hydroxymethylpyrimidine and 5-(2-hydroxyethyl)-4-methylthiazole. The pyrimidine is further metabolized, first to 2-methyl-4hydroxy-5-hydroxymethylpyrimidine followed by 2-methyl4-hydroxy-5-pyrimidine-carboxylic acid. The thiazole metabolites 4-methylthiazole-5-acetic acid and 2-oxyd...

In Salmonella typhimurium, thiamine is a required nutrient that is synthesized de novo. Labeling studies have demonstrated probable precursors for both the 4-amino-5-hydroxymethyl-2-methylpyrimidine pyrophosphate moiety and the 4-methyl-5-(beta-hydroxyethyl) thiazole monophosphate moiety. The isolation of thiamine auxotrophs with mutations in at least five different genetic loci is reported. Th...

Starting from epothilone A±D (1a±2b) obtained by large scale fermentation of the myxobacterium Sorangium cellulosum the thiazole side-chain was extensively modi®ed by substitution, oxidation and replacement. Metallation afforded the C-19 carbanion 4 which was quenched by various carbon and heteroatom electrophiles to give C-19 substituted epothilones 5. Thiazole N-oxides 9 were obtained by trea...