Concentrations of mono- and divalent cations in the testis, three different regions of the epididymis and the vas deferens of lizard, M. carinata were determined. The concentrations of Na+, K+ and Ca2+ increased gradually from testis to vas deferens. However, no significant variation in the levels of Mg2+ was observed between testis and anterior epididymis, and between posterior epididymis and ...

Yeast phenylalanine tRNA (tRNAPhe) exhibits a 4$-fold enhancement of fluorescence in response to the addition of Mg2+. Concurrently, over the same concentration range (0 to 0.4 m&r) of Mg2+, the circular dichroism peak at 263 nm displays a 20% increase in ellipticity and a blue shift of 2 nm. The existence of the negative circular dichroism band at 295 nm appears to be cation-dependent. This co...

Yeast phenylalanine tRNA (tRNAPhe) exhibits a 4$-fold enhancement of fluorescence in response to the addition of Mg2+. Concurrently, over the same concentration range (0 to 0.4 m&r) of Mg2+, the circular dichroism peak at 263 nm displays a 20% increase in ellipticity and a blue shift of 2 nm. The existence of the negative circular dichroism band at 295 nm appears to be cation-dependent. This co...

Interaction with divalent cations is of paramount importance for RNA structural stability and function. We report here a detailed molecular dynamics study of all the possible binding sites for Mg2+ on an RNA duplex, including both direct (inner sphere) and indirect (outer sphere) binding. In order to tackle sampling issues, we develop a modified version of bias-exchange metadynamics, which allo...

to membranes in brain and liver was similar to, but more marked than, that observed with Mg2.+ (Table 2). This can be explained on the basis of a possible interaction between cations and the negatively charged groups on the endoplasmic-reticulum membranes. Brain endoplasmic reticulum has considerably more phospholipid than liver, hence the requirement for spermine and Mg2+ may also be greater i...

The binding of Mg2+ to the ribitol teichoic acid of Staphylococcus aureus H walls was examined by equilibrium dialysis in solution and in the intact wall; the influence of alanyl ester groups on binding was determined. In solution the ribitol polymer had a lower affinity than did a glycerol teichoic acid and bound Mg2+ in the ratio Mg2+/P of 1:1. The presence of alanyl ester residues caused a d...

The binding of cations to ATP-G-actin has been assessed by measuring the kinetics of the increase in fluorescence of N-acetyl-N'-(5-sulfo-1-naphthyl)-ethylenediamine-labeled actin. Ca2+ and Mg2+ compete for a single high-affinity site on ATP-G-actin with KD values of 1.5-15 nM for Ca2+ and 0.1-1 microM for Mg2+, i.e. with affinities 3-4 orders of magnitude higher than previously reported (Fried...

Intracellular levels of the divalent cations Ca2+ and Mg2+ are important regulators of cell cycle and proliferation. However, the precise mechanisms by which they are regulated in cancer remain incompletely understood. The channel kinases TRPM6 and TRPM7 are gatekeepers of human Ca2+/Mg2+ metabolism. Here, we investigated the human neuroblastoma cell line SHEP-21N in which the MYCN oncogene (en...

Cost-effective and controllable synthesis of M0.25Ni0.15Cu0.25Co0.35Fe2O4 (M2+ = Mg2+, Mn2+, Cd2+) ferrites via the sol–gel auto-combustion technique. The impact divalent cations on structural, dielectric, optoelectrical properties was examined by XRD, FTIR, Raman, LCR, UV–Vis, two probe I-V measurement techniques. crystallite size 52.66 nm, minimum specific surface area observed 5.1507 m2/g fo...

Salinization of groundwater in semiarid regions, as a limiting factor of use, has a strategic importance. In this study, the sources of salinity, chemistry and Quality of groundwater in Robat – Khorramabad plain have been identified using geochemical methods. Using data analysis, the concentration of cations and anions are recognized as Ca2+>Na+ >Mg2+>K+ and HCO3-> Cl-> SO42+> NO3-> F-. High co...