Cadmium (Cd) is a testicular toxicant and endocrine disruptor. In the present study, we investigated the effects of maternal Cd exposure during the late pregnant period on testicular development and steroidogenesis in male offspring. Pregnant mice were injected intraperitoneally with CdCl(2) (0.5mg/kg) daily from gestational day (gd) 13 to gd 17. As expected, fetal weight and crown length were ...

Neospora caninum and Toxoplasma gondii are related parasites. The former is a common cause of abortion in dairy cattle. The latter has not been conclusively demonstrated in bovine fetuses. During the course of attempts to isolate N. caninum from aborted fetuses, T. gondii was isolated from 2 aborted fetuses, 1 from Portugal and 1 from the United States. Both isolates were made by bioassay of fe...

T1Wh (Rb (5;19)1Wh), an albino strain of mice not previously used in the investigation of cleft palate (CP), is homozygous for a (5;19) Robertsonian translocation which provides conspicuous chromosomal markers. Preliminary studies in this laboratory revealed that CP is induced in all the offspring of T1Wh females treated with 2.5 mg of cortisone acetate on days 11-14 of pregnancy and that palat...

In order to analyze the genetic and physiological basis of controls affecting the generation of the repertoire of antigen-binding cells in fetal mice, we have measured the numbers of spleen cells specific for each of four antigens as a function of the total numbers of nucleated and Ig-bearing cells in inbred, hybrid, and random bred fetuses. For each of the two inbred strains BALB/c and CBA/J, ...

background: the aim of the study was to provide a point of reference to study the neospora caninum infections in china. methods: genome dna was extracted from the brains of aborted fetuses and specific pcr was performed with n. caninum nc5-targeted specific primers. fetal bovine brain tissues were homogenized and continuously cultured in vero cells with double antibodies. the medium was replace...

miconazole is an imidazole antifungal agent, commonly applied topically to the skin or mucous membranes. the aim of this study was to examine the alternative method for gaining mechanism or the bimolecular changes caused by the possible teratogenic effects of miconazole on mice fetus brain tissue using ftir-microspectroscopy. the mice were injected with miconazole (60 mg/kg) on gestation day 9....

BACKGROUND Rodent models of abdominal wall defects (AWD) may provide insight into the pathophysiology of these conditions including gut dysfunction in gastroschisis, or pulmonary hypoplasia in exomphalos. Previously, a Scribble mutant mouse model (circletail) was reported to exhibit gastroschisis. We further characterise this AWD in Scribble knockout mice. METHOD Homozygous Scrib knockout mic...

metronidazole is used to treat trichomoniasis, bacterial vaginosis, and other diseases. there are controversy aspects about its teratogenicity. a teratogenic agent can alter morphology or subsequent function of the fetus. the aim of this study was to examine an alternative method for the recognition of the mechanism or the bimolecular potential changes in mice fetus caused by metronidazole usin...

The effects of ethanolic extract from the seeds of Annona squamosa were studied in gestating ICR mice subcutaneously treated with 4 mg/kg BW mercuric chloride (MA) and their fetuses in utero starting GD 14 16. Effects of solo exposure of A. squamosa at 25 mg/kg BW or its combinatorial exposure at 25 mg/kg BW and 75 mg/kg BW with MA in the maternal mice and their fetuses were evaluated at GD 19....

We developed pancreatic and duodenal homeobox1 (Pdx1) knockout mice to improve a compensatory hyperinsulinemia, which was induced by hyperplasia in the β cells or Langerhans' islands, as the diabetic model mice. For targeting of Pdx1 gene by homologous recombination, ES cells derived from a 129(+Ter) /SvJcl×C57BL/6JJcl hybrid mouse were electroporated and subjected to positive-negative selectio...