There is increasing interest in interfacing dissociated neuronal cultures with planar multielectrode arrays (MEAs) for the study of the dynamics of neuronal networks. Here we report on the successful use of three-dimensional tip electrode arrays (3D MEAs), originally developed for use with brain slices, for recording and stimulation of cultured neurons. We observed that many neurons grew direct...

Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting from accumulated biomolecular mass. As an example, 50 spots for each of four proteins consisting o...

Protein microarray technology has made enormous progress in recent years. It has been successfully applied for the identification, quantification and functional analysis of proteins in basic and applied proteome research. Protein microarrays have the potential to replace singleplex analysis systems. A variety of different analytical platforms have been developed that are likely to evolve into k...

With the advancement of metagenome data mining science has become focused on microarrays. Microarrays are datasets with a large number of genes that are usually irrelevant to the output class; hence, the process of gene selection or feature selection is essential. So, it follows that you can remove redundant genes and increase the speed and accuracy of classification. After applying the gene se...

Background: Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder in women. The abnormalities of endocrine and intra-ovarian paracrine interactions may change the microenvironment for oocyte development during the folliculogenesis process and reduce the developmental competence of oocytes in PCOS patients who are suffering from anovulatory infertility and pregnancy loss....

Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.   Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated aga...

Background Although spermatozoa delivers its RNA to oocytes at fertilization, its biological role is not well characterized. Our purpose was to identify the genes differentially and exclusively expressed in sperm samples both before and after the swim-up process in control donors and infertile males with the purpose to identify their functional significance in male fertility. MaterialsAndMethod...

Acetylcholinesterase (AChE) is responsible for hydrolyzing the acetylcholine neurotransmitter, bringing an end point to cholinergic neurotransmission. Thus, AChE primary target of a wide spectrum compounds used as pesticides, nerve agents or therapeutic drugs neurodegenerative diseases such Alzheimer’s disease (AD). This enzyme heterogeneously distributed in brain showing different activity dep...