A field experiment was conducted to illustrate the different degree and dynamics of microbial community structure and function in the rhizosphere across four growing stages (before plantation and three growth stages) using a combination of biochemical (enzyme assay and microbial biomass carbon) and molecular approaches of qPCR and PCR-DGGE (polymerase chain reaction-denaturing gradient gel elec...

Manure applications can benefit crop productivity by adding required nutrients and organic matter to soil. There is a paucity of information on how soil microbial community dynamics will be altered by the application of manure to different landscape positions. Thus, an in situ field study was conducted during the summer and winter months to evaluate microbiological properties of three soil type...

One Bacillus thuringiensis (Bt) and two stacked Bt and cowpea trypsin inhibitor (Bt + CpTI) cottons and their non-transgenic isolines were consecutively cultivated to investigate the soil persistence of Cry1Ac and CpTI proteins and their effects on microbial properties and enzyme activities involving C, N, P, and S cycling in soil. Results showed that there were the persistence of Cry1Ac and Cp...

We examined the effect of decomposing leaf litter and dissolved inorganic nutrients on the heterotrophic biofilm of submerged wood in streams with and without leaves. Leaf litter was excluded from one headwater stream in August 1993 at Coweeta Hydrologic Laboratory in the southern Appalachian Mountains. We compared microbial processes on wood in the litter-excluded stream to a reference stream ...

Uracil phosphoribosyltransferase (UPRT) is a pyrimidine salvage pathway enzyme that catalyzes the conversion of uracil to uridine monophosphate (UMP). The enzyme is highly conserved from prokaryotes to humans and yet phylogenetic evidence suggests that UPRT homologues from higher-eukaryotes, including Drosophila, are incapable of binding uracil. Purified human UPRT also do not show any enzymati...