Sodium polyanethol sulfonate (SPS) at 500 microgram/ml, but not sodium amylosulfate (SAS) at 500 microgram/ml, precipitated egg white lysozyme (1 mg and 50 microgram of lysozyme per ml) as determined with the assay strain Micrococcus lysodeikticus ATCC 4698. Fresh and heat-inactivated (56 degrees C, 30 min) human serum (80%, vol/vol) killed M. lysodeikticus (10(4) bacteria per ml at zero time) ...

The first total synthesis of marine-derived penicimonoterpene (±)-1 has been achieved in four steps from 6-methylhept-5-en-2-one using a Reformatsky reaction as the key step to construct the basic carbon skeleton. A total of 24 new derivatives of 1 have also been designed and synthesized. Their structures were characterized by analysis of their 1H NMR, 13C NMR and HRESIMS data. Some of them sho...

The promoters of Micrococcus luteus, a bacterium whose chromosomal DNA has a high G + C content (74%), diverge from the consensus prokaryotic promoter in having GC-rich DNA sequences at less important positions (Nakayama, M., Fujita, N., Ohama, T., Osawa, S., and Ishihama, A. (1989) Mol. Gen. Genet. 218, 384-389). In order to compare the promoter selectivity of RNA polymerase between M. luteus ...

The impact of steroid hormones, and particularly estradiol, on human microbiota could be recognized as a substantial part human-microbiota interactions. However, an area that remains poorly investigated is the skin vaginal microbial communities biofilms, which contain non-pathogenic bacteria phyla Firmicutes Actinobacteria, especially probiotic genus Lactobacillus widespread, safe genus, Microc...

Four bacteria, identified as Pseudomonas aeruginosa, Alcaligenes eutrophus, Bacillus subtilis and Micrococcus luteus were isolated from crude oil polluted soils using anthracene as the sole carbon and energy source. All the organisms utilized n-hexadecane, n-tetradecane, diesel oil, engine oil and naphthalene as sole carbon sources. None could utilize hexane, cycloheptane, xylene, benzene, tolu...

Fermented succulent shoots of bamboo (Bambusa balcooa and Dendrocalamus strictus) are an enriched source of phytosterol. Microorganisms from the ‘soibum exudate’ involved in microbial bioconversion of phytosterol during fermentation of succulent bamboo shoots were isolated and identified as Bacillus subtilis, B. licheniformis, B. coagulans and Micrococcus luteus. Crude phytosterol was purified ...

More than 70 years ago, Alexander Fleming discovered lysozyme and proposed that nonpathogenic bacteria fail to cause disease because they are very susceptible to destruction by lysozyme, an enzyme that is one of the principal proteins of phagocytes. Although much has been learned about the effects of lysozyme in vitro, its biological role in vivo has not been determined. We examined transgenic ...

A recent report from this laboratory (Kingkade, Huff, and Behrens, 1948) (Iescribes experiments in which lysozyme was used to prepare bacterial cell lysates from Staphylococcus aureus K1 for use as a possible medium for bacteriophage multiplication. It was hoped that the cell wall would be ruptured allowing dispersal of the intracellular constituents into the medium without appreciable denatura...

A bioreactor with a column of flocculated cells of the moderate halophile Micrococcus varians subsp. halophilus which adsorbed the halophilic nuclease H was designed to be used in the production of 5' nucleotides from RNA. A remarkable characteristic of the flocculated cells was that they preferentially adsorbed much exogenous nuclease, excluding adsorbed 5' nucleotidase. Furthermore, desalting...