We generated a high-density genetic linkage map of soybean using expressed sequence tag (EST)-derived microsatellite markers. A total of 6920 primer pairs (10.9%) were designed to amplify simple sequence repeats (SSRs) from 63,676 publicly available non-redundant soybean ESTs. The polymorphism of two parent plants, the Japanese cultivar 'Misuzudaizu' and the Chinese line 'Moshidou Gong 503', we...

The pfcrt allelic type and adjacent microsatellite marker type were determined for 82 Plasmodium falciparum isolates from the Philippines. Mutant pfcrt allelic types P1a and P2a/P2b were dominant in different locations. Microsatellite analysis revealed that P2a/P2b evolved independently in the Philippines, while P1a shared common ancestry with Papua New Guinea chloroquine-resistant parasites.

In order to examine the population structures of the red fox (Vulpes vulpes) on the Hokkaido Island in Japan, we conducted analysis on 250 foxes from all over the island for 12 microsatellite loci. Assignment tests using the genotype data set showed that they were divided into 6 subpopulations. Of the 6, one was geographically isolated in the southern region and considered definitive subpopulat...

Intraspecific competition is a pervasive phenomenon with important ecological and evolutionary consequences, yet its effect in natural populations remains controversial. Although numerous studies suggest that in many cases populations across all organisms are limited by density-dependent processes, this conclusion often relies on correlative data. Here, using an experimental approach, we examin...

The present study was undertaken to isolate and genotype Toxoplasma gondii from free-range chickens (Gallus domesticus) from villages in Maharashtra and Tamil Nadu states of central and south India, respectively. Blood, heart, and brain from a total of 741 chickens were examined for T. gondii infection. Antibodies to T. gondii, as assayed with the modified agglutination test (MAT >or = 1:5) wer...

Forests and woodlands are under continuing pressure from urban and agricultural development. Tree-dependent mammals that rarely venture to the ground are likely to be highly sensitive to forest fragmentation. The Australian squirrel glider (Petaurus norfolcensis) provides an excellent case study to examine genetic (functional) connectivity among populations. It has an extensive range that occur...

Leišová-Svobodová L., Tomková L., Sedláček T., Psota V., Kučera L. (2014): The application of microsatellite analysis in barley malting quality breeding programmes. Czech J. Genet. Plant Breed., 50: 268–277. A set of 43 microsatellite loci was used to characterise the malting barley varieties and breeding lines used for the Protected Geographical Indication (PGI) “České pivo” (“Czech beer”) and...

The greater ani (Crotophaga major), a Neotropical cuckoo, exhibits an unusual breeding system in which several socially monogamous pairs lay eggs in a single nest and contribute care to the communal clutch. Cooperative nesting is costly-females compete for reproduction by ejecting each other's eggs-but the potential direct or indirect fitness benefits that might accrue to group members have not...

Williams-Beuren syndrome (WBS) is a rare genetic disorder (1/20,000-50,000) and is usually caused by a 1.5- to 1.8-Mb heterozygous deletion on chromosome 7q11.23. At least 25 genes have been identified in the deletion region in WBS patients, which is flanked by large low-copy-repeat sequences (> 320 kb). By using FISH as well as microsatellite analysis, it is not possible to get a precise ident...

Centre for Plant Health/BioMarka, School of Agriculture and Food Systems, Faculty of Land and Food Resources, The University of Melbourne, Parkville, Victoria, Australia 3010 School of Forestry and Ecosystem Science, Faculty of Land and Food Resources, The University of Melbourne, Parkville, Victoria, Australia 3010 Current Address: Department of Primary Industries-Bundoora, Victoria, Australia...