With an ultra-high surface area and abundant functional groups, graphene oxide (GO) provides an ideal substrate for the immobilization of trypsin. We demonstrated that trypsin could be immobilized on GO sheets assisted by polymers as molecular spacers to maintain the activity of the enzyme. And with the trypsin-linked GO as the enzyme immobilization probe, a novel microwave-assisted on-plate di...

Sample preparation is the critical step of any analytical protocol, and involves steps from simple dilution to partial or total digestion. The present review focuses on wet digestion methods used for solid and liquid sample pre-treatment. The methods include wet decomposition and dissolution of the organic and inorganic samples, in open and closed systems, using thermal and radiant (ultraviolet...

The best enzyme to prepare peanut peptides, papain, coupled with microwave irradiation was selected from five common proteases according to the results of the yield of peanut peptides [nitrogen solution index (NSI) in trichloroacetic acid (TCA), TCA-NSI] and the degree of hydrolysis (DH). The main factors that influenced the microwave-coupled enzymatic digestion method were optimized by respons...

Proteomics will contribute greatly to the understanding of gene functions in the post-genomic era. In proteome research, protein digestion is a key procedure prior to mass spectrometry identification. During the past decade, a variety of electromagnetic waves have been employed to accelerate proteolysis. This review focuses on the recent advances and the key strategies of these novel proteolysi...

High pressure and temperature were combined with the convenient mixture of acids to perform an effective digestion of samples from aquatic animals for phosphorus (P) analysis. Our results indicate that digestion in high-pressure bombs combined with concentrated acidic media can be a convenient way to yield all P stored in samples, and to produce accurate recoveries. Phosphorus recovery of a cer...

A microwave plasma was used for direct ambient ionization mass spectrometry of solid substrates, rapidly yielding atomic spectra without sample digestion or pre-treatment. Further, molecular spectra for the organic components of the substrate were obtained simultaneously, in an ambient ionization format. Initial characterization of the microwave plasma coupling to an ion trap mass spectrometer ...