METHODS: Nineteen vials of tetrofosmin were radiolabelled with Tc and the percentages of the RCP were determined. Five different methods were compared with the standard RCP testing method (ITLC-SG, 2x20 cm): Whatman 3MM (1x10 cm) with acetone and dichloromethane (method 1); Whatman 3MM (1x10 cm) with ethyl acetate (method 2); aluminum oxide-coated plastic thin-layer chromatography (TLC) plate (...

The initiation of osteogenesis in the mandibular mesenchyme of the embryonic chick at 7 days is dependent upon an epithelial induction which occurs in the mandible up to the fourth day in ovo. In the present study, transfilter tissue recombinations were used to study this inductive mechanism. The epithelial and mesenchymal components of the mandibles were separated before the completion of the ...

Western Blot Membranes were immunoblotted with the following primary antibodies: rabbit polyclonal anti-Lmp7 (1:2000; AbD Serotec); rabbit polyclonal anti-β2M (1:1000; Proteintech); mouse anti β-actin (1:30000; Chemicon); mouse anti importin-β; (1:5000; Millipore); mouse anti-acetylated tubulin (1:1000; Millipore); mouse anti β-tubulin (1:1000; Millipore); rabbit anti-ERK (K23, 1:1000; Santa Cr...

The bones of the head and face of vertebrate embryos only form after their progenitor cells have undergone an inductive interaction with embryonic epithelia. We have investigated whether epithelial cell products can substitute for epithelia in allowing mandibular ectomesenchyme to form bone. Mandibular epithelia from embryonic chicks were cultured on Millipore filters for 28 days to allow them ...

Pseudomonas aeruginosa strains were grown in 1-cm plastic chambers sealed at both ends with porous Millipore filters and implanted in the peritonea of mice. Mucoid and nonmucoid strains of P. aeruginosa isolated from a patient with cystic fibrosis largely retained their phenotypes when grown for up to 1 year in this in vivo system, although colonial dissociation occurred, as observed in chronic...

BACKGROUND Identification of the source of mosquito blood meals is an important component for disease control and surveillance. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling has emerged as an effective tool for mosquito blood meal identification, using the abdomens of freshly engorged mosquitoes. In the field, mosquito abdomens a...

Chalcroft, S. C. W., Gavin, J. B., and Seelye, R. N. (1974). Thorax, 29, 539-544. A method for evaluating the effects of ailograft sterilization procedures on the viability of human fibroblasts. A semiquantitative method for assessing the effects of allograft sterilization procedures on the viability of cultured human fibroblasts is described. The proportions of 'viable', 'altered', and 'non-vi...

A membrane filtration technique with commercially available membrane filters (Millipore Corp.) was effective for the removal of Reiter treponemes from liquids such as fluorescent-antibody conjugates, to which the organisms are added for adsorption. Reiter treponemes from an 8-day culture were not microscopically detectable in filtrates through membranes with a pore diameter of 0.45 mum, but tre...

Periodate-oxidized ppp(A2'p)3A was coupled in high yield to an acyl hydrazide derivative of bovine serum albumin and the conjugate was used to immunize rabbits. Several potent and specific antisera were obtained, and one was tested extensively. Fifty per cent of the bound probe ppp(A2'p)3A3'[32P]pCp was displaced by compounds containing the moiety -pA2'pA-at concentrations of 20-60 nM and by co...

The sensitivity of PCR for the amplification of target nucleic acid sequences in clinical diagnostics may often be reduced due to the presence of inhibitory factors. Hemolytic serum contains a number of PCR inhibitors, one of which is hemin. In this study we have found that conventional methods of DNA extraction were not sufficient for the removal of PCR-inhibitory compounds in hemolytic serum....