A molecular-based detection method was developed to detect Escherichia coli O26, O111 and O157 in minced (ground) beef samples. This method consists of an initial overnight enrichment in modified tryptone soya broth (mTSB) and novobiocin prior to DNA extraction and subsequent serogrouping using a triplex PCR. This method has a low limit of detection and results are available within 24 hours of ...

Different culture media were used to detect enterotoxigenic Staphylococcus aureus strains from peptone salt solution and inoculated minced meat. When recovery, reactions, and counting mistakes were considered, it was concluded that Baird-Parker agar was the best medium.

the quality control of microbial contamination in meats consumed in restaurants of university of tehran was taken into consideration. for this reason, 100 sterile samples were taken from 4 central restaurants, from may until october 1995, the samples were as follows: 12 samples of meat, 50 samples of chopped meat and 38 samples of minced meat. all of the samples were examined to search for meso...

Impact of antimicrobials, naturally produced by lactic acid bacteria, on the Listeria monocytogenes growth in minced salmon | Joana Šalomskienė, Dovile Jonkuvienė, Irena Mačionienė, Raimundas Narkevičius, Reda Riešutė Agricultural Journals

The objective of the present study was undertaken to standardize the levels of shredded potato and added water in the development of chevon cutlets. The lean meat was minced twice through 4 mm plate. The cutlets were prepared with the incorporation of shredded potato at 0, 5, 10 and 15% level and added water at 0, 3, 6 and 9%, respectively replacing the lean meat. A significant effect of mincin...

D-values were obtained for Listeria monocytogenes and Yersinia enterocolitica at 50, 55 and 60 degrees C in vacuum-packed minced beef samples heated in a laboratory water-bath. The experiment was repeated using vacutainers, which allowed heating of the beef to the desired temperature before inoculation. D-values of between 0.15 and 36.1 min were obtained for L. monocytogenes. Pre-heating the be...

A method is presented for isolation of 2-10 gm of highly puri fied nucleoli from Walker tumors. Initially, 0.5-1 kg of tumor was minced in a specially devised stainless steel mincer. The minced tissue was then homogenized in a continuous tissue homogenizer in 0.25 Msucrose containing 3.3 HIMCaClj. The nuclear preparation, obtained by continuous flow centrifugation, was subjected to sonic oscill...

This article reports on an investigation into the ability of minced banana peel to extract lead and copper ions from water and the parameters involved in this process. The kinetics of copper and lead uptake reached equilibrium in 10 min and the extraction of metals ions was favorable above pH 3. The medium was characterized by FTIR, which showed absorption bands of carboxylic and amine groups a...