We investigated phylogenetic relationships among five species of Japanese brown frogs by the analysis of nucleotide sequences in the cytochrome b gene of mitochondrial DNA (mtDNA). The sequence of the 251-base pairs, which cover approximately 22% of the cytochrome b gene, was determined by PCR-Direct sequencing method. Phylogenetic relationships were analyzed by UPGMA, neighbor-joining, maximum...

This is the first time that direct PCR - DNA amplification without prior DNA extraction - was successfully developed and fully validated for rapid and economical simultaneous identification of six commonly consumed meat species. To achieve this, six species-specific primers were selected from previous reports and newly designed from the mitochondrial cytochrome b (cyt b), cytochrome oxidase I (...

The genus Bionychiurus Pomorski, 1996 is not recorded from India so far. A new species nominated as tamilensis sp. n. described the Nilgiri Hills in Western Ghats. possess a dorsal pso formula 32/133/33,343 and ventral 11/011/11,121. similar to changbaiensis, B. normalis, oblongatus reported China Korea. 658 bp mitochondrial cytochrome oxidase subunit I DNA region (COX1) of was sequenced for it...

B-acute lymphoblastic leukemia (B-ALL) is the frequent pediatric malignity. Chemotherapy is the most practical approaches to deal with such malignancies. Microtubule-targeted agents are one of the most strategic drugs which formerly use in chemotherapy.Although,colchicine-binding anti-tubulin agents exhibited promising effects in clinical trials, their exact mechanism of action is not fully und...

The Southern smooth snake, Coronella girondica, is a small-sized colubrid found in Northwest Africa and Southwest Europe. Mitochondrial DNA-based studies showed that the species can be split into five clades: two from (one Moroccan one Tunisian-Algerian) three Europe south-west of Iberian Peninsula, south-east Spain rest European range). With regards to Italy, date, only samples have been analy...

To reconstruct the phylogenetic position of the extinct cave lion (Panthera leo spelaea), we sequenced 1 kb of the mitochondrial cytochrome b gene from two Pleistocene cave lion DNA samples (47 and 32 ky B.P.). Phylogenetic analysis shows that the ancient sequences form a clade that is most closely related to the extant lions from Africa and Asia; at the same time, cave lions appear to be highl...

The DNA barcode of Bruchidius varius collected from Dilek Peninsula and Büyük Menderes Delta National Park (DPNP) in Aydın province Turkey is given for the first time. As barcode, mitochondrial cytochrome c oxidase subunit I (COI) with a length approximately 628 bp sequenced an illustrative presented along nucleotide composition target gene location. Nucleotide sequence includes 38.63% T, 27.98...

Alpers-Huttenlocher syndrome (AHS) is an uncommon autosomal recessive mitochondrial DNA depletion disease. The classic clinical triad of progressive developmental regression, liver degeneration, and seizures helps define the disorder, but a wide range expressions occur. most common mutations in childhood have been identified cytochrome c oxidase Ⅰ Ⅳ genes. 7706G˃A missense mutation Cox Ⅱ gene w...

Cytochrome b, coded by mitochondrial DNA, is one of the cytochromes involved in electron transport in the respiratory chain of mitochondria. Cytochrome b is a critical intermediate in a mitochondrial death pathway. To reveal whether cytochrome b of the mosquito Aedes aegypti (L.) (Diptera: Culicidae) (AeaCytB) is developmentally regulated, we used real-time quantitative polymerase chain reactio...