background: due to some limitations of serological methods in diagnosis of patients infected with hiv-1 (human immunodeficiency virus) and hcv (hepatitis c virus), it is profoundly important to use molecular methods for the detecting of these infectious agents. however, the most significant problems are the exorbitant cost of these methods and the need of a thermocycler which is an expensive in...

Detection and monitoring of target species is the primary strategy in management control biological invasions. Traditional methods to detect invasive are time consuming cumbersome, with a requisite for trained taxonomists identification aquatic species. Environmental DNA (eDNA)-based molecular offer an alternative, as they quick, cost-effective require minimal manpower. In this study, we design...

Objective(s): First, 10-bromodecanoic acid was covalently attached to all available surface primary amines of PPI G2 and G3 to increase their lipophilicity. In the subsequent step, PPIs were conjugated to the alkylcarboxylate groups of alkylcarboxylate-PPI derivatives to increase the number of surface primary amines. Physicochemical properties of modified PPIs were determined. Transfection expe...

The aim of present study was to compare the diagnostic performance of the different Brucella abortus antigen based serological and molecular tests such as Rose Bengal Plate Test (RBPT), indirect enzyme linked immunosorbent assay (I-ELISA) and polymerase chain reaction (PCR). Out of 89 samples, 44 were positive by I-ELISA, 18 by RBPT and 21 by PCR. Substantial agreement was observed between PCR ...