patients and methods in this cross-sectional study, peripheral mononuclear cells (pbmcs) were isolated from peripheral blood of 29 patients with newly diagnosed pulmonary tb and 19 patients with positive tuberculin skin test. the pbmcs were activated with ppd for 72 hours. activated cells were harvested, rna was extracted and cdna was synthesized. a real-time taqman method was designed and opti...

The concentration of IgG and IgA was measured in the supernatants of peripheral blood mononuclear cells and of cells harvested from the intestinal lamina propria, which were cultured in vitro in the presence or absence of mitogens. The lamina propria mononuclear cells were harvested by collagenase digestion of macroscopically normal mucosa from 10 fresh surgical resections for carcinoma. Secret...

Numerous cell types are involved in maintenance of the intestinal tissue. However, the main players are cells of the epithelial lining and the immune system. Human peripheral blood mononuclear cells (PBMCs) are used to investigate the effect of food bioactives on various immune cells. These cells are easily isolated from blood of healthy donors or buffy coats (leukocyte concentrates, a by-produ...

Background: NK (natural killer) and NKT (natural killer T) cells, as components of innate immune system, play a crucial role in tumor progression and dissemination. Objective: To investigate the percentages of NK cells, NKT cells, iNKT (invariant natural killer T) cells, total T lymphocytes as well as activated T lymphocytes, in tumor draining lymph nodes (TDLN...

The study of pathogenic systems involved in multiple sclerosis (MS) is essential for the development new therapies. Objective : to determine contribution B-lymphocytes production IL-17 MS with an assessment ability B-cells induce differentiation Th17 and own by this pathology. Material methods . A total 81 subjects were examined, 68 whom patients diagnosed MS, 13 healthy individuals. concentrat...

The chemotactic migration in vitro of peripheral blood, intestinal mucosal, and mesenteric lymph node mononuclear cells has been assessed in patients with colorectal carcinoma. Peripheral blood mononuclear cells of patients exhibited normal chemotaxis. For control patients with non-malignant, non-inflammatory intestinal disease, the chemotaxis of mucosal mononuclear cells was similar to that of...