background: this study quantified the effect of four popular culture media in a biodiesel production study on the qualitative and quantitative lipid content, dry biomass, and lipid productivity of nannochloropsis oculata. methods: culture of microalgae was done separately in walne, f/2, sato, and tmrl media. in the logarithmic and stationary growth phases, biomass production and lipid accumulat...

conclusions according to the results of the present study, it can be concluded that adipose derived mscs can be cultured in serum-free media with no change in their differentiating capacity. this finding gives us a hope for future cell therapy studies and trials with little concern about zoonotic infections or immunological reaction. results the isolated cells showed adherent spindle shaped mor...

Four monoclonal antibodies, XT-I, MT-23, MT-24 and MT-29, that bind the XT-1-differentiation-antigen of male germ cells have been used to investigate the biological role of the XT-1-molecule of germ cells in short-term primary culture. Cultures from 10 days postpartum mice demonstrate increasing numbers of antigen-positive germ cells and increased antigen expression per cell with succeeding day...

introduction: cyclosporin a (cya) is a potent immunosuppressive drug widely used in transplant settings due to its specific inhibition of t–cell activation. several studies have reported the synergistic effect of cya with hematopoietic growth factor in colony culture of mice bone marrow. we studied the effect of il-3, il-6, scf,cya and nutrition medium ( condition medium - pha) on the developme...

Abstract. Suminar E, Budiarto R, Nuraini A, Mubarok S, Ezura H. 2022. Morpho-physiologicalresponsesof iaa9 tomato mutants to different levels of PEG simulated drought stress. Biodiversitas 23: 3115-3126.This study aimed analyze the morphological and physiological responses its WT-MT stress in vitro growing medium. This applied a completely randomized design with two factors, i.e., genotypes pol...

We recently found that a human T-cell leukemia virus type 1-infected cell line, MT-2, could support the replication of hepatitis C virus (HCV) (N. Kato, T. Nakazawa, T. Mizutani, and K. Shimotohno, Biochem. Biophys. Res. Commun. 206:863-869, 1995). In order to develop a culture system in which HCV replicates more efficiently, we examined the efficiency of HCV replication in cloned MT-2 cell lin...

large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as fi lling materials. the aim of this study was to evaluate the cytotoxic effect of a new copaiba oil-based root canal sealer (biosealer [bs]) on osteoblast-like osteo-1 cells. materials and methods: the experimental groups were formed according to the culture medium cond...

The aim of the present study was to investigate the effect of recombinant Mycobacterium tuberculosis (r-Mt) 10-kDa co-chaperonin (cpn10) on the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL) in third-generation cultured osteoblasts. The osteoblast-like cultures were isolated from bone fragments taken from patients undergoing surgery. Prior to stim...

Hepatic stellate (HS) cells were isolated from the livers of metallothionein (MT)-null and control mice and used to establish IMS/MT(-) and IMS/N cell lines, respectively, using SV40 virus transformation. Cellular morphology, incorporation of vitamin A and expression of alpha-SMA, desmin and SV40 T-antigen were used to confirm that both cell lines were immortal HS cells. The growth rates of bot...