Purpose: To develop a novel detection technology for drug-resistance mutation sites of Mycobacterium tuberculosis (MTB) using Luminex liquid chip.Methods: Using polymerase chain reaction (PCR) amplification and hybridization analysis, MTB infection drug-resistant the first-line second-line anti-MTB drugs were simultaneously identified. A method was applied to analyze wild-type standard strains ...

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Mycobacterium tuberculosis (Mtb) coinfection has been observed in a number of nations it is connected with illness death. paper studies reaction–diffusion within-host Mtb/SARS-CoV-2 model immunity. This explores the connections between uninfected epithelial cells, latently Mtb-infected productively SARS-CoV-2-infected free Mtb...

A previous unbiased genome-wide analysis of CD4 Mycobacterium tuberculosis (MTB) recognition using peripheral blood mononuclear cells from individuals with latent MTB infection (LTBI) or nonexposed healthy controls (HCs) revealed that certain MTB sequences were unexpectedly recognized by HCs. In the present study, it was found that, based on their pattern of reactivity, epitopes could be divide...

BACKGROUND Previous studies have shown that Mycobacterium tuberculosis (MTB) Uganda family, a sub-lineage of the MTB Lineage 4, is the main cause of tuberculosis (TB) in Uganda. Using a well characterized patient population, this study sought to determine whether there are clinical and patient characteristics associated with the success of the MTB Uganda family in Kampala. METHODS A total of ...

Metal-carboxylic ionophore complexes, where Metal = Ag, Tl, Li, Na, K, Rb, Mg, Mn, Cs, Sr, Ba, Cu and Ionophore = monensin (MON-H), have been synthesized. The molecular structures of three complexes have been solved by X-ray diffraction methods. The metal chelates were tested against human tuberculosis line for potential anti-tubercular activity against Mycobacterium tuberculosis H37Rv (MTB). T...

OBJECTIVE Understanding human immunity to Mycobacterium tuberculosis (Mtb) during different stages of infection is important for development of an effective tuberculosis (TB) vaccine. We aimed to evaluate immunity to Mtb infection by measuring immune responses to selected Mtb antigens expressed during different stages of infection over time and to observe sustainability of immunity. METHODS I...

Defining the mechanisms of Mycobacterium tuberculosis (Mtb) persistence in the host macrophage and identifying mycobacterial factors responsible for it are keys to better understand tuberculosis pathogenesis. The emerging picture from ongoing studies of macrophage deactivation by Mtb suggests that ingested bacilli secrete various virulence determinants that alter phagosome biogenesis, leading t...

The Mycobacterium tuberculosis dihydrodipicolinate synthase (Mtb-dapA) is an essential gene. Mtb-DapA catalyzes the aldol condensation between pyruvate and L-aspartate-beta-semialdehyde (ASA) to yield dihydrodipicolinate. In this work we tested the inhibitory effects of structural analogues of pyruvate on recombinant Mtb-DapA (Mtb-rDapA) using a coupled assay with recombinant dihydrodipicolinat...

Purpose Mycobacterium tuberculosis (Mtb) bacilli have been found in retinal pigment epithelial (RPE) cells from uveitis patients without signs of systemic tuberculosis (TB) infection. RPE cells are important for ocular immune privilege and uveitis development. Methods To address a potential role for Mtb-infected RPE cells in the development of uveitis, we delineated the response to Mtb infect...

Background: To evaluate the performance of Xpert MTB/RIF assay for direct detection of Mycobacterium tuberculosis (Mtb) and rifampin resistance in pulmonary and extrapulmonary tuberculosis (TB) patients in China. Material/Methods: We prospectively enrolled 506 TB patients to evaluate sensitivity and specificity of Xpert MTB/ RIF by comparing with Mtb culture, fluorescent staining smear microsco...