Abstract. Sayono, Nurullita U, Handoyo W, Tyasningrum WS, Chakim I, Budiharjo A. 2023. Bioassay and molecular detectionsof insecticides resistance of Aedes aegypti, vector dengue in Central Java Province, Indonesia. Biodiversitas 24: 300-307. The emergence insecticide-resistant strains among aegyptipopulations hampered Dengue control programs the endemic areas. Moreover, to understand current s...

background : apolipoprotein e (apoe) gene encodes an important protein in reforming injuries of central nervous system (cns). it is assumed that various apoe alleles may be functionally different. the purpose of this study was to investigate the distribution of apoe genotypes in multiple sclerosis (ms) patients in a small cohort of iranians. methods : in this case-control study, blood samples o...

different research groups have extensively studied the associations of cytokine gene polymorphisms in different diseases. the role of cytokines gene polymorphisms in multiple sclerosis (ms), as a chronic immune-mediated neurodegenerative disease, has been previously reported in the various populations. for determining pro-inflammatory cytokine gene polymorphisms, 100 relapsing remitting multipl...

Conventional and molecular techniques were applied to detect and characterize drug resistance of mycobacteria in the sputum samples of clinically confirmed tuberculosis. The sensitivities of mycobacterium detection by ZN staining, culture, multiplex PCR, and restriction fragment length polymorphism (RFLP) were 27.7%, 19.9%, 92.9%, and 95.7%, respectively, but all were 100% specific. The convent...

Polymerase chain reaction-amplified product length polymorphism (PCR-APLP) is one of the most convenient and reliable methods for single nucleotide polymorphism (SNP) analysis. This method is based on PCR, but uses allele-specific primers containing SNP sites at the 3'-terminus of each primer. To use this method at least two allele-specific primers and one "counter-primer", which serves as a co...

We develop a strategy for haplotype analysis of PCR products that contained two adjacent heterozygous loci using sequencing with specific primers, allele-specific primers, and ddNTP-blocked primers. To validate its feasibility, two sets of PCR products, including two adjacent heterozygous SNPs, UGT1A1⁎6 (rs4148323) and UGT1A1⁎28 (rs8175347), and two adjacent heterozygous SNPs, K1637K (rs1117601...

The polymerase chain reaction (PCR) has been applied in a novel manner to detect the multiple mutations causing cystic fibrosis (CF). PCR-mediated site-directed mutagenesis (PSM) has been applied to create allele-specific restriction enzyme cutting sites for three of the more common mutations. Two other mutations after cutting sites on their own. We discuss the implications for the expedient de...