The capability to fabricate nanoscale structures is a fundamental step toward realizing the promise and potential of nanotechnology. We report on precise manipulation and thermal processing using 100-nm polystyrene latex nanoparticles. This approach is illustrated by fabricating a three-dimensional nanostructure by using an AFM tip to position nanoparticles and then thermally processing to "sin...

BACKGROUND Chromosomal dissection provides a direct advance for isolating DNA from cytogenetically recognizable region to generate genetic probes for fluorescence in situ hybridization, a technique that became very common in cyto and molecular genetics research and diagnostics. Several reports describing microdissection methods (glass needle or a laser beam) to obtain specific probes from metap...

BACKGROUND Condensin is thought to contribute to large-scale DNA compaction during mitotic chromosome assembly. It remains unknown, however, how the complex reconfigures DNA structure at a mechanistic level. RESULTS We have performed single-molecule DNA nanomanipulation experiments to directly measure in real-time DNA compaction by the Xenopus laevis condensin I complex. Condensin can bind to...