We evaluated the Roche LightCycler Staphylococcus M(GRADE) kits to differentiate between Staphylococcus aureus and coagulase-negative staphylococci in blood cultures growing clusters of gram-positive cocci. Testing 100 bottles (36 containing S. aureus), the assay was 100% sensitive and 98.44% specific for S. aureus and 100% sensitive and specific for coagulase-negative staphylococci.

The isolation of two coagulase-negative strains of Staphylococcus pyogenes is reported, and their biochemical characteristics are given. The reliability of coagulase tests alone in the identification of Staph. pyogenes is discussed, and the deoxyribonuclease test is recommended as a useful confirmatory test.

The crescents were accompanied by foci of fibrinoid necrosis with endocapillary and extracapillary fibrin. Tubular atrophy and interstitial fibrosis were absent. Interstitial inflammation was present, focal and accompanied by tubular degenerative changes. There was evidence of necrotizing vasculitis. IF revealed granular, segmental to global glomerular capillary wall positivity for IgG, kappa a...

The accuracy and efficiency of Staf-Sistem 18-R (Liofilchem s.r.l., Roseto degli Abruzzi, Teramo, Italy) were compared with those of conventional biochemical methods to identify 523 strains belonging to 16 different human Staphylococcus species. Overall, 491 strains (93.9%) were correctly identified (percentage of identification, > or = 90.0), with 28 (5.4%) requiring supplementary tests for co...

The objective of this study was to evaluate the prevalence of coagulase-negative staphylococci in healthy dogs and to determine whether methicillin-resistant staphylococci expressed the mecA gene. Nasal and rectal swab samples were taken from 50 clinically healthy dogs. The prevalence of coagulase-negative staphylococci was evaluated according to phenotypic properties. The agar diffusion method...

PURPOSE The aim of this study was to compare the resistance patterns of bacteria in vitreous fluid from patients undergoing vitrectomy for diagnostic reasons, with bacteria of other nosocomial infections. METHODS Vitreous fluid samples (n=144) were obtained from 133 patients undergoing vitrectomy for endophthalmitis, and 11 for uveitis as suspected endophthalmitis. They were Gram stained and ...

This study was carried out to determine the prevalence of sub-clinical mastitis, its etiological agents and their antibiogram in Murrah buffaloes at an organized farm. A total of 326 quarter milk samples were screened from 82 apparently healthy buffaloes. The percent prevalence of sub-clinical mastitis was found to be lower on the basis of SCC (>5x105/ml) alone (23.17) as compared to cultural e...