Methods are described for rearing large quantities of Ditylenchus dipsaci on alfalfa tissues. Nematodes and alfalfa seed were disinfected and nematodes were reared in quantities sufficient to provide a continuous supply of inoculum for our alfalfa-breeding program. Nematodes reproduced best in darkness at 20-25 C. Cultures reached maximum numbers in 3-6 wk.

Pratylenchus vulnus (L, L, adults) are attracted to the roots of growing bean seedlings. The attraction is inhibited by treating the nematodes with solutions of carbofuran or phenamiphos at concentrations below those necessary to inhibit motility and dispersion. Nematicide treatments of plants without treatment of nematodes are ineffectual because nematodes are attracted equally well to treated...

Nematodes are tiny but complex unsegmented roundworms that are anatomically differentiated for feeding, digestion, locomotion, and reproduction. These small animals occur worldwide in all environments. Most species are beneficial to agriculture. They make important contributions to organic matter decomposition and to the food chain. Some species, however, are parasitic to plants or animals. Pla...

Root-knot nematodes and cyst nematodes are obligate, biotrophic pathogens of numerous plant species. These organisms cause dramatic changes in the morphology and physiology of their hosts. The molecular characterization of induced plant genes has provided insight into the plant processes that are usurped by nematodes as they establish their specialized feeding cells. Recently, several gene prod...

A field experiment was conducted to investigate the soil application of two Rhizobacteria, (MTCC and Pf-5) and urea treatments, separately and in combination on free living and plant parasitic nematodes. All the treatments significantly decreased the number of plant-parasitic nematodes with the increasing of plant growth. Both Rhizobacteria and urea were separately effective in suppressing plan...

Exposure to carbofuran and fenamiphos for 72 hours reduced the numbers of active Aphelenchus avenae in aqueous suspension by > 75%. When nematicides were removed, many A. avenae exposed to carbofuran resumed normal movement but A. avenae treated with fenamiphos did not recover. Acetylcholinesterase (AChE) activity was suppressed by > 95% in nematodes treated with carbofuran or fenamiphos. Howev...

Spliced leader trans-splicing occurs in many primitive eukaryotes including nematodes. Most of our knowledge of trans-splicing in nematodes stems from the model organism Caenorhabditis elegans and relatives, and from work with Ascaris. Our investigation of spliced leader trans-splicing in distantly related Dorylaimia nematodes indicates that spliced-leader trans-splicing arose before the nemato...

results significant differences were observed in the results of co-culture of nematodes larva and fungi after seven days. the most effective fungi against the nematodes larvae were cladosporium sp., trichoderma sp., fusarium equisetti, after seven days of incubation. materials and methods sheep feces were employed to provide the required third stage larvae source for the experiments. the nemato...

From 1990 through 1992, nematodes were extracted from soil samples taken from the rhizosphere of native species of grapes from four areas of northern California and two areas of southern California. For comparison, samples from domestic grapes as well as a putative hybrid of Vitis califarnica and V. vinifera were also taken. Rhizosoil from California native grapevine contained many more species...

A checklist of nematode parasites from Indonesian murids with their geographic distribution is presented. This checklist is compiled from three sources: the catalogue of nematode parasites of Museum Zoologicum Bogoriense (unpublished specimens in the collection), data from our previous research and articles on nematodes of Indonesian murids. This checklist is presented as a list of nematode par...