Background: Circoviruses are small, non-enveloped, single stranded DNA viruses. There is scarce information about these agents in non-psittacine birds. Aims: It is attempted to detect and characterize circoviruses in non-psittacine birds. Methods: Forty-five samples were collected from different non-psittacine species belonging to seven avian o...

A rapid and sensitive reverse transcription polymerase chain reaction (RT-PCR) and nested-PCR were used to detect bovine viral diarrhea virus 1 (BVDV-1) in bull semen. Selected primers could amplify a part of the 5´UTR of the BVDV genome. A 294 bp DNA fragment was amplified and specificity of the results was confirmed by direct sequencing of the PCR product. Prior to RNA extraction, the seminal...

Several methods for the detection of Borna disease virus (BDV) RNA have been reported, one being the reverse transcription-nested polymerase chain reaction (RT-nested PCR) method. However, due to the possibility of contamination of the cloned DNA in a reaction tube, false-positive results might be obtained by RT-nested PCR. To detect only BDV RNA without anxiety of contamination, we developed a...

Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. In the present study, the surface modified cadmium-telluride...

OBJECTIVE To investigate the prevalence of herpes simplex virus (HSV), cytomegalovirus (CMV), and Epstein-Barr virus (EBV) in the semen of men with fertility problems. DESIGN A descriptive clinical study. SETTINGS Outpatient infertility clinic of a private hospital. PATIENT(S) One hundred thirteen men attending an infertility clinic in Athens. INTERVENTION(S) Semen samples were collecte...

Introduction: Respiratory syncytial virus (RSV) is one of major causes of viral respiratory tract disease in young children and infants. We aimed to study the frequency of respiratory syncytial virus and its subtypes in pneumonia and bronchopneumonia in pediatric patients less than two years old tested by nested polymerase chain reaction with the evaluation of routinely used methods of diagnosi...

We report the first intestinal infections in Tunisia with Entamoeba moshkovskii in two healthy adults. Entamoeba moshkovskii cysts were distinguished from those of the morphologically identical parasites Entamoeba histolytica and Entamoeba dispar by specific nested polymerase chain reaction and sequencing.