The urgent need of neuraminidase inhibitors (NI) has provided an impetus for understanding the structure requisite at molecular level. Our search for selective inhibitors of neuraminidase has led to the identification of pharmacophoric requirements at various positions around acyl thiourea pharmacophore. The main objective of present study is to develop selective NI, with least toxicity and dru...

A comparison was made between the amounts of sialic acid released from viable cell suspensions of normal and malignant cells by Vibrio cholerae neuraminidase (VCN) and by influenza virus neuraminidase. More sialic acid can be removed by enzyme digestion from various tumors than from normal lymphoid cells. More sialic acid can be removed from the surface of both normal and tumor cells by VCN tha...

Understanding how influenza viruses traverse the mucus and recognize host cells is critical for evaluating their zoonotic potential, prevention treatment of disease. The surface A virus covered with receptor-binding protein hemagglutinin receptor-cleaving enzyme neuraminidase, which jointly control interactions between cell. These proteins are organized in closely spaced trimers tetramers to fa...

Whole saliva specimens from eight healthy human adults were examined for neuraminidase. The presence of two types of neuraminidase in four samples out of eight was demonstrated by means of polyacrylamide gel electrophoresis and sucrose density gradient centrifugation. One type is soluble and the other an insoluble, perhaps particle-bound, enzyme. The pH optima were 5.8 for the former and 5.0 to...

Influenza antiviral agents play important roles in modulating disease severity and in controlling pandemics while vaccines are prepared, but the development of resistance to agents like the commonly used neuraminidase inhibitor Tamiflu may limit their future utility. We report here a completely new class of specific, mechanism-based anti-influenza drugs that function via the formation of a high...

Two enzyme forms of alkaline phosphatase have been partially purified from the medium spent for the culture of HUH-6 clone 5 cells, which were originally derived from hepatoblastoma tissue. The purification methods used are ammonium sulfate precipitation, ethanol precipitation, diethylaminoethyl cellulose chromatography, Affi-Gel Blue chromatography, and Sephadex G-200 gel filtration. These alk...

It has been known that the agglutinability of human red cells is changed or enhanced by treatments with proteolytic enzymes or neuraminidase. In this paper, the serological properties of agglutinogens developed by proteolytic enzymes (bromelin, ficin, papain, trypsin and pronase) and neuraminidase are investigated by using antisera to trypsin- and neuraminidase-treated red cells. The adsorption...

= Abstract = Human placental glutathione S-transferase(GST 7[ ) was purified to the apparent homgeneity through salting-out with ammonium sulfate and the consecutive chromatography on carboxymethyI(CM)-, diethylaminoethyI(DEAE)-cellulose and S-hexylglutathione sepharose 6B affinity column. For the characterization of the apparently purified enzyme, sodium dodecyl sulfate polyacrylamide gel elec...

The Y155H amino acid substitution in the neuraminidase gene (NA) has previously been associated with highly reduced inhibition by neuraminidase inhibitors in the seasonal H1N1 influenza A virus which circulated in humans before the 2009 pandemic. During the 2012/13 epidemic season in Spain, two A(H1N1) pdm09 viruses bearing the specific Y155H substitution in the NA were detected and isolated fr...