The electrophoresis pattern of apoptotic cells detected by the comet assay has a characteristic small head and spread tail. This image has been referred to as an apoptotic comet, but it has not been previously proven to be apoptotic cells by any direct method. In order to identify this image obtained by the comet assay as corresponding to an apoptotic cell, the frequency of appearance of apopto...

AIM Despite its therapeutic value almost nothing is known about potential adverse health effects of Olea europea L. We therefore investigated the in vitro toxicity and genotoxicity of leaf extracts of this plant. MATERIAL AND METHODS Extracts from olive tree leaves were obtained from four different regions in Tunisia. We investigated the in vitro toxicity, genotoxicity and antigenotoxicity of...

background: many studies have been published on the antioxidative effects of boric acid (ba) and sodium borates in in vitro studies. however, the boron (b) concentrations tested in these in vitro studies have not been selected by taking into account the realistic blood b concentrations in humans due to the lack of comprehensive epidemiological studies. the recently published epidemiological stu...

Background: This study has used biomarker of exposure-effect continuum to examine the biological characteristics of organophosphate (OP) toxicity and its genotoxic effect among rice farmers. Materials and Methods: A cross-sectional study was conducted among 160 pesticide exposed rice farmers and 160 adults from the fishing village as the unexposed group. They share the common socio-economical ...

The micronucleus (MN) test and the alkaline single cell gel or comet assay were applied to exfoliated cells of the buccal mucous in order to evaluate the genotoxic risk associated with occupational exposure of 10 storage battery renovation workers, and 10 car painters, with age matched controls, in Pelotas, RS, in southern Brazil. In the MN test, 2000 exfoliated buccal cells were analyzed for e...

Sperm cryopreservation is widely used for both research and reproduction purposes, but its effect on sperm DNA damage remains controversial. Sperm DNA fragmentation (SDF) has become an important biomarker to assess male infertility. In particular, the differentiation between single- and double-stranded DNA fragmentation (ssSDF and dsSDF) has clinical implications for male infertility where ssSD...

BACKGROUND Arsenic trioxide and 1,25-(OH)2D3 (vitamin D3) are used for the treatment of lymphocytic leukemia. However, the effects of combined treatment of these drugs are controversial. In this study, the combined effects of these drugs on the induction of apoptosis in NB4 cells were investigated using the neutral comet assay. METHODS NB4 cells were treated with various doses of arsenic trio...