Newcastle disease virus (NDV) and Salmonella Pullorum have significant damaging effects on the poultry industry, but no previous vaccine can protect poultry effectively. In this study, a recombinant-attenuated S. Pullorum strain secreting the NDV hemagglutinin-neuraminidase (HN) protein, C79-13ΔcrpΔasd (pYA-HN), was constructed by using the suicide plasmid pREasd-mediated bacteria homologous re...

The appearance of very virulent strains of infectious bursal disease (IBD) virus at the end of the 1980s made it necessary to develop more effective immunization procedures. To facilitate this, the immunogenicity and the immunosuppressive effect of a mild (G-87), an intermediate (LIBD) and an intermediate-plus (IBDV 2512) IBDV strain were tested after the in ovo inoculation of 18-day-old SPF an...

In an effort to protect chickens against Newcastle disease (ND), a nonreplicating virosome vaccine was produced by solubilization of Newcastle disease virus (NDV) with Triton X-100 followed by detergent removal with SM2 Bio-Beads. Biochemical analysis indicated that the NDV virosomes had similar characteristics as the parent virus and contained both the fusion and hemagglutinin-neuraminidase pr...

Vaccination programs to control Newcastle disease include use of low virulent live virus vaccines or inactivated vaccines to induce protective immunity. In order to further characterize the immune response elicited by live virus, we evaluated both cellular and humoral immune response to Newcastle disease virus (NDV) consequent to vaccine application by oculonasal and intranasal routes. Specific...

Newcastle Disease (ND) is an acute, highly contagious viral disease in poultry. Despite the intensive vaccination programs to control Newcastle Disease Virus (NDV), the recent infection of NDV in Iran has led to severe morbidity and mortality in Chukar Partridges. The aim of this study was to investigate the ability of Inactivated Oil Adjuvant Vaccine (IOAV) following use of live vaccine to hav...

Commercial chickens with a high level of maternal antibodies for Newcastle disease were vaccinated when newly hatched with Queensland V4 or Ulster 2C Newcastle disease virus (NDV) strains by nebulization. The exposure time to a fine aerosol of vaccine produced with an ultrasonic nebulizer was 60 sec. The chickens were challenged oculonasally with virulent NDV strain Texas GB in weekly intervals...

background: newcastle disease virus (ndv) is the causativeagent of the newcastle disease (nd), a highly contagiousdisease in birds that causes significant economic losses to thepoultry industry worldwide. nd is endemic in iran and outbreaksare reported regularly in commercial poultry flocks and differentspecies of birds. objectives:the current study was carried outto characterize ndv based on p...