1. Enzyme distribution between chloroplasts and the nonchloroplast parts of green leaf cells of Spinacia oleracea, Nicotiana rustica, Vicia faba, and Phaseolus vulgaris have been investigated by use of the nonaqueous chloroplast isolation technique. Whereas pyruvate kinase and peroxidase were located only or mainly outside of the chloroplasts, the other enzymes studied, isocitric dehydro genas...