The Yes-associated protein (YAP) is a potent transcriptional co-activator that functions as a nuclear effector of the Hippo signaling pathway. YAP is oncogenic and its activity is linked to its cellular abundance and nuclear localisation. Activation of the Hippo pathway restricts YAP nuclear entry via its phosphorylation by Lats kinases and consequent cytoplasmic retention bound to 14-3-3 prote...

introduction to define the clinical features, biochemical and histological findings and outcome of three forms of autoimmune hepatitis.   materials and methods in a cross sectional study between november 2001 to january 2008 in tehran and mashhad university of medical sciences, 61 children who diagnosed as aih (40 girls and 21 boys) have been analyzed for their clinical, serological, and histol...

background: vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. methods: simian rotavirus sa11 gene 4 cdna was cloned into a cloning plasmid pdonrtm by recombination reaction using clonase ii enzyme mix. the resulting clone was c...

We are developing a new method for protein structure determination that overcomes limitations in traditional methods, such as x-ray crystallography or nuclear magnetic resonance, and requires about a thousand fold less protein. The method, called Antibody Imprinting, uses antibodies against target proteins and random peptide probe libraries to map the epitopes of antibody binding sites on targe...

The remodeling of nuclear organization during differentiation and the dramatic alteration of nuclear organization associated with cancer development are well documented. However, the importance of tissue architecture in the control of nuclear organization remains to be determined. Differentiation of mammary epithelial cells into functional tissue structures, in three-dimensional culture, is cha...

Sera can produce nuclear or perinuclear immunofluorescence staining in neutrophils which may be caused by antibodies with differing antigenic specificities. These include perinuclear antineutrophil cytoplasmic antibodies (P-ANCA), granulocyte specific antinuclear antibody (GS-ANA), and antinuclear antibody (ANA). There is controversy over the value of formalin fixation of neutrophils in differe...

  Background and Objectives:  The molecular genetic of the human herpes virus 8 (HHV8) has now been characterized the pathogenesis of kaposi sarcoma (KS). This study attempted to determine the rate of HHU-8 infection in KS in an Iraniann cross sectional study. Method & material: In this cross-sectional study, we used paraffin-embedded specimens of 54 clinically well-characterized kaposi sarcom...

Using nuclear envelopes from Chinese hamster ovary (CHO) cells as an antigen, a mouse monoclonal antibody (IgM; designated mAb CHON211) that specifically binds to components of the nuclear pore complex (nucleoporins) was isolated. Immunofluorescence localization of the antigen recognized by mAb CHON211 revealed a punctate pattern restricted to the nuclear envelope; this pattern changed dramatic...

Background: Graves’ disease is an autoimmune disease, characterized by the presence of antibodies directed to TSH receptor or nearby regions as well as antibodies to double strands DNA (dsDNA) anticardiolipin and nuclear antibodies. This study evaluated anticardiolipin and rheumatoid factor, such as IgA and IgM antibodies in patients with Graves’ disease. Patients and methods: Anticardiolipin a...

Background: Apart from genetic and environmental factors, activation of autoreactive mechanisms has been proposed to play a role in the pathogenesis of schizophrenia. In re-cent years, considerable work has been carried out to understand the role and contribution of the immune system in this disease. Objective: To investigate the T cell response to phytohaemagglutinin (PHA) and determine the se...