The high frequency backscatter from cells with a nucleus to cell volume ratio of 0.50 cannot be adequately modeled as a homogeneous sphere. It was hypothesized that the cytoplasm of such cells is of fluid nature. This work attempts to model the ultrasound backscatter (10-62 MHz) from some non-nucleated biological specimens. This was done by measuring the backscatter response from individual sea...

The effects of cell concentration during cryopreservation on bone marrow (BM) or peripheral blood (PB)-derived hematopoietic progenitor cells have not been described. The much greater numbers of cells harvested for autologous PB stem cell (PBSC) transplantation requires that the cells be frozen at higher cell concentrations, or in much greater volumes, compared with BM. We cryopreserved 108 PBS...

An analysis of NMDP data shows that allele-level matching for HLA A, B, C, and DRB1 is preferred in the selection of adult unrelated donors. If mismatching is unavoidable, mismatches at HLA B or C may be better tolerated than those at A or DRB1. Whether mismatches are at the allele level (ie, within an antigen group) or at the antigen level makes no difference in outcome, except at HLA C where ...

The membrane components of rabbit bone-marrow-bound erythroid cells were characterized and compared with those of circulating rabbit erythroid cells. By the criteria of sodium dodecyl sulphate/polyacrylamide-gel electrophoresis, radioiodination with lactoperoxidase and binding of radioiodinated lectins, the two circulating forms of erythroid cells (the reticulocyte and erythrocyte) have the sam...

OBJECTIVES To comparatively analyze maternal and fetal factors and quality markers of blood samples in a public umbilical cord blood bank. METHOD This is a cross-sectional descriptive study that revisited 458 records of donations from September 2009 to March 2013 at the Hemocentro de Santa Catarina. The means of markers were used to define cutoff points for the quality of cord blood. RESULT...

Enucleation of L cells leads to loss of the capacity to produce interferon, showing that the cell nucleus is essential for interferon formation. However, when the cells were enucleated while interferon formation. However, when the cells were enucleated while interferon formation was proceeding, the cytoplasts were capable of continuing to synthesize interferon by a process shown to be protein s...

There is an error in the 10 sentence of the first paragraph in the Enrichment of putative CTCs from patients with metastatic breast and lung cancer section of the Results. The correct sentence is: This population varied in distribution across all samples, and was present at an average proportion of 48.8615.5% of the total nucleated cells (Table S2 in File S1). Table S2 in File S1 is incorrect. ...

The effects of constant exposure to high concentrations of methotrexate in vivo on the committed stem cell (CFU-C) were studied by in vitro culture of mouse bone marrow. Bone marrow samples were obstained from animals receiving a continuous infusion, and were cultured in a methotrexate-free semisolid gel system. The effects of methotrexate infusion on the pluripotent stem cell population (CFU-S...

Over the years, with the advancement in hematology analyzer technology, the use of fluid analysis method has seen a drastic increase in clinical examinations. Cell counting and classification in independent body fluid analysis method are conducted by semiconductor laser flow cytometry and nucleic acid fluorescence staining techniques. This study is to evaluate the efficacy of Sysmex XN-1000 hem...