A previously isolated rice (Oryza sativa) rbcS gene was further characterized. This analysis revealed specific sequences in the 5' regulatory region of the rice rbcS gene that are conserved in rbcS genes of other monocotyledonous species. In transgenic rice plants, we examined the expression of the beta-glucuronidase (gusA) reporter gene directed by the 2.8-kb promoter region of the rice rbcS g...

BACKGROUND Small animal models have been previously used in transfusion medicine studies to evaluate the safety of blood transfusion products. Although there are multiple studies on the effects of blood banking practices on human red blood cells (RBCs), little is known about the effect of blood component manufacturing on the quality of rat RBCs. METHODS Blood from Sprague-Dawley rats and huma...

The conversion of hemoglobins (Hbs) and red blood cells (RBCs) from the larval to the adult type was monitored during normal metamorphosis in Xenopus laevis, and in artificially induced metamorphosis-arrested and precociously metamorphosed animals by means of SDS-PAGE, Hb immunohistochemistry, and double-staining with in situ DNA nick-end labeling (TUNEL) for detection of apoptosis and Hb immun...

BACKGROUND We have showed that secretory Apolipoprotein J/Clusterin (sCLU) is down-regulated in senescent, stressed or diseased red blood cells (RBCs). It was hypothesized that sCLU loss relates to RBCs vesiculation, a mechanism that removes erythrocyte membrane patches containing defective or potentially harmful components. METHODOLOGY/PRINCIPAL FINDINGS To investigate this issue we employed...

The carbon intensity of China’s resource-based cities (RBCs) is much higher than the national average due to their relatively intensive mode development. Low transformation RBCs an important way achieve goal reaching emissions peak in 2030. Based on panel data from 116 China 2003 2018, this study takes opening high-speed railway (HSR) lines as a quasi-experiment, using time-varying difference-i...

background: investigation of toxicity of silver nanoparticle, especxially blood toxicity, is necessary because this nanoparticle is used a lot in diffrerent parts of life and invironment. the aime of this is evaluation the effects of silver nanoparticles with different concentrations on blood cells pregnant wistar rats. materials and methods: in this case control study, 30 pregnant rat were div...

To evaluate the relationship between aquaporin-1 (AQP1) expression and the cell volume of red blood cells (RBCs), canine peripheral RBCs were separated according to specific gravity, and expression of the AQP1 protein on the membrane of RBCs was compared using anti-dog AQP1 polypeptide serum. Western blot analysis indicated that there was no significant difference in AQP1 expression between lar...

BACKGROUND Animal models are valuable in transfusion research. Use of human red blood cells (RBCs) in animal models facilitates extrapolation of the impact of storage conditions to the human condition but may be hampered by the use of cross species. METHODS Investigation of clearance and posttransfusion recovery in a rat model using fresh and stored human RBCs. RESULTS Directly following tr...