We present an object-level metric for segmentation performance which was developed to quantify both overand under-segmentation errors, as well as to penalize segmentations with larger deviations in object shape. This metric is applied to the problem of segmentation of cell nuclei in routinely stained H&E histopathology imagery. We show the correspondence between the metric terms and qualitative...

In this work, we present an automated method for the detection and boundary determination of cells nuclei in conventional Pap stained cervical smear images. The detection of the candidate nuclei areas is based on a morphological image reconstruction process and the segmentation of the nuclei boundaries is accomplished with the application of the watershed transform in the morphological color gr...

Segmentation-free direct methods are quite efficient for automated nuclei extraction from high dimensional images. A few such methods do exist but most of them do not ensure algorithmic robustness to parameter and noise variations. In this research, we propose a method based on multiscale adaptive filtering for efficient and robust detection of nuclei centroids from four dimensional (4D) fluore...

Image segmentation is a basic technology in the field of image processing and computer vision. Medical an important application plays increasingly role clinical diagnosis treatment. Deep learning has made great progress medical segmentation. In this paper, we proposed Residual-Attention UNet++, which extension UNet++ model with residual unit attention mechanism. Firstly, improves degradation pr...

This paper describes a multi-resolution technique to combine diagnostically important visual information at different magnifications in H&E whole slide images (WSI) of gastric cancer. The primary goal is to improve the results of nuclei segmentation method for heterogeneous histopathological datasets with variations in stain intensity and malignancy levels. A minimum-model nuclei segmentation m...

We propose an unsupervised statistical region based active contour approach integrating an original fractional entropy measure for image segmentation with a particular application to single channel actin tagged fluorescence confocal microscopy image segmentation. Following description of statistical based active contour segmentation and the mathematical definition of the proposed fractional ent...

Overlapping of cervical cells and poor contrast of cell cytoplasm are the major issues in accurate detection and segmentation of cervical cells. An unsupervised cell segmentation approach is presented here. Cell clump segmentation was carried out using the extended depth of field (EDF) image created from the images of different focal planes. A modified Otsu method with prior class weights is pr...

MOTIVATION Our goal is to develop a screening platform for quantitative profiling of colony organizations in 3D cell culture models. The 3D cell culture models, which are also imaged in 3D, are functional assays that mimic the in vivo characteristics of the tissue architecture more faithfully than the 2D cultures. However, they also introduce significant computational challenges, with the main ...

Protozoan parasites cause many diseases, such as malaria, EHEC infection, shigellosis, amoebiasis, etc. Different kinds and growing stages of protozoan parasites would lead to different treatments. The most significant characteristic of different growing stages is the number of nuclei. But some nuclei in a cell could be unclear causing the missing in nucleus detection.Common and traditional seg...