Objective: Investigated the hypolipidemic effect of alcoholic extract Moringa oleifera seeds using zebrafish hyperlipidemia model.
 Methods: Using 0.3% egg yolk solution to feed larval construct model. These fish in model were treated with an for 2 days. Then, tail vessels observed after oil red O staining, and accumulated optical density lipids was analyzed. The body length, triglyceride ...

conclusions based on our findings, we conclude that the separation and reproduction of adipose tissue cells is an appropriate method for purification of mscs in animal studies. regarding the histomorphometric and flow cytometry analysis results, we demonstrated the differentiation ability of mscs in normal medium and hope to employ these cells for the regeneration of damaged bone tissues in the...

We describe a 16-year-old boy with rhabdomyosarcoma, consistent with embryonal subtype, of the lower extremity who received systemic neoadjuvant chemotherapy and subsequent excision. Microscopic sections of the postchemotherapy excision demonstrated diffuse, prominent, and immature adipocyte-like differentiation, in addition to skeletal muscle differentiation. Adipocyte-like differentiation was...

Lipoid pneumonia is an unusual form of pneumonitis. It is categorized as either exogenous or endogenous, depending on the lipid source. Diagnosis requires a high index of suspicion, with clues provided by history and characteristic radiographic findings. Evaluation of samples obtained via bronchoscopy can confirm the diagnosis with oil red O staining, without requiring surgical biopsy. We repor...

BACKGROUND The clinical benefits of lipid lowering with statins are attributed to changes in plaque composition leading to lesion stability, but supporting clinical data from human studies are lacking. Therefore, we investigated the effect of 3 months of pravastatin treatment on composition of human carotid plaques removed during carotid endarterectomy. METHODS AND RESULTS Consecutive patient...

PURPOSE To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials...

In this study, we fed yellow drum juveniles (initial body weigh 5.57 ± 0.03 g) a fish-oil-based, soybean-oil-based (SO), or berberine chloride (BBR 50 mg kg-1 in SO-based diet) diet with three replicates of each treatment for 56 days. Dietary BBR supplementation significantly increased growth performance but decreased SO-induced viscerosomatic index and condition factor increases. Hepatic tissu...

This study aimed to culture and characterize mesenchymal stem cells derived from meniscal debris. Cells in meniscal debris from patients with meniscal injury were isolated by enzymatic digestion, cultured in vitro to the third passage, and analyzed by light microscopy to observe morphology and growth. Third-passage cultures were also analyzed for immunophenotype and ability to differentiate int...

OBJECTIVE The current study was to explore the effect of melatonin on osteoporosis and relevant mechanisms. MATERIALS AND METHODS We performed micro-CT to detect bone microstructure and ELISA to detect the contents of osteocalcin (OCN) and bone alkaline phosphatase (BAP) in serum. Double fluorescence labeling of calcein and tetracycline and toluidine blue staining were used to determine morph...

Paper electrophoresisiswidely used in clinicalmedicine as a screening test for hyperlipidemia. It is a simple, reproducible procedure, but requires 36 h to complete. Aliquota of sera are electrophoresed 16-18 h, paper strips are dried for 30 mm, followedby overnightstaining(1, 2). Recently it was reported that stainingtime could be decreased to 2 h by using Oil Red 0 stain in acetone-water solv...