The underlying idea of oligonucleotide conjugates is to provide oligonucleotide with some novel property [...].

Temperature-controlled nanoelectrospray ionization explores the thermodynamics of hybrid quadruplex–duplex oligonucleotide assemblies comprising multiple domains and brings insight into mutual domain interaction. As described by Renato Zenobi et al. in their Research Article on page 15390, thermal denaturation experiments were conducted to reveal unfolding intermediates changes DNA complexes de...

Abstract Genome-editing tools from Oligonucleotide-Directed Mutagenesis (ODM) to CRISPR system use synthetic oligonucleotides for targeted exchange of nucleotides. Presently, majority genome-editing protocols are dependent on the in vitro cell or tissue culture systems with somaclonal variation, and limitations plant regeneration. Therefore, here, we report an alternative planta cellular test o...

Oligonucleotide assisted tri(8-hydroxyquinoline) aluminium (Alq3) microrods were prepared for the first time. When hybridized with oligonucleotide labeled by Cy3 fluorescent dye, a significant photoluminescence variation of the Alq3 microrods was observed due to Förster resonance energy transfer, unlike when Cy5-oligonucleotide was used. Versatile nucleotide manipulation would open up wider app...

18.1 The Challenge of Delivery of Oligonucleotide Analogs and siRNA into Cells in Culture and In Vivo ............................................ 313 18.2 Chemical Synthesis of Peptide–Oligonucleotide Conjugates .............. 314 18.2.1 Synthesis of CPP Conjugates to Phosphate-Containing Oligonucleotides ...................................................................... 315 18.2.2 Synthes...

Synthetic oligonucleotide probes can be easily obtained and used, in contrast to cDNA cloning to develop probes, and thus the present study was carried out to determine whether such probes could also be useful for in situ hybridization. A 24-base synthetic oligonucleotide complementary to part of the alpha-melanocyte-stimulating hormone (alpha-MSH) coding region of proopiomelanocortin (POMC) mR...