In this paper an exact penalty line search is introduced for solving constrained nonlinear programing. By this method it is possible to solve some problems with combining penalty method and SQP method, and in any iteration for producing new stepone can uses linear search and solves unconstrained optimization by exact penalty function. In this method a linear programming subproblem with trust ar...

The polymerase chain reaction (PCR) is used widely for the in vitro amplification of DNA. The versatility of PCR can be enhanced by the introduction of sequential steps such as nested primer amplifications (1) or re-amplification protocols (2). PCR can also be done on cDNA templates produced by reverse transcription of RNA (3). These protocols require additional opening and closing of the react...

otitis media with effusion (ome) is one of the most common causes of hearing loss (hl) in children. it has been reported that several factors such as eustachian tube dysfunction, insufficiencies in the aeration of the mastoid cells, allergies, immunity, and infections play an important role in the etiology of the disease. little is known about the role of helicobacter pylori (h. pylori) in extr...

A rapid and simple method, termed multiplex mutagenically separated PCR (MS-PCR), was developed to detect several molecular defects in the hemoglobin gene in one PCR. This technique, in which different-size allele-specific primers were used, specifically amplified both normal and mutant alleles of the globin gene in the same reaction. Subsequent gel electrophoresis showed at least one of the tw...

Amplification of a 3-kb genome region from the RNA polymerase gene to the 3' poly(A) tail of small round-structured virus (SRSV) by reverse transcription-PCR (RT-PCR) has been difficult to achieve because of a stable secondary structure in a region between the RNA polymerase gene and the 5' end of the second open reading frame. We have developed a one-tube RT-PCR method to efficiently amplify t...

In clinical situations where a diagnostic real-time PCR assay is not sensitive enough, leading to low or falsely negative results, or where detection earlier in a disease progression would benefit the patient, an unbiased pre-amplification prior to the real-time PCR could be beneficial. In Amp-PCR, an unbiased random Phi29 pre-amplification is combined with a specific real-time PCR reaction. Th...

Attempts are frequently made to semiquantitate mRNA as a means of circumventing the laborious and time-consuming process of quantitation that is inherent in the use of competitor templates. However, semiquantitative approaches present the risk of generating non-reproducible data due to tube-to-tube variability and/or misinterpretation of quantities of product being generated during the plateau ...