It is well established that the structural integrity of mammalian taste buds is dependent on an intact innervation . This is based on the following lines of evidence . Experiments in which gustatory nerves were severed resulted in degeneration of taste buds (5, 8, 9, 14, 17) . If gustatory nerves were allowed to regenerate into the areas they previously innervated, taste buds reappeared (8, 10,...

Because of its low oxygen consumption, about 0.1 &/mg./hr., the ocular lens has been considered as a metabolically inert tissue. However, any interference in the carbohydrate metabolism results in the opacification of this normally transparent organ. Therefore, it appears that metabolism is essential for the viability of the lens. That a consumption of oxygen, even if low, is also necessary was...

Because of its low oxygen consumption, about 0.1 &/mg./hr., the ocular lens has been considered as a metabolically inert tissue. However, any interference in the carbohydrate metabolism results in the opacification of this normally transparent organ. Therefore, it appears that metabolism is essential for the viability of the lens. That a consumption of oxygen, even if low, is also necessary was...

PURPOSE To determine whether suppression of flow may be detrimental to trabecular cell survival and to the morphologic characteristics of the trabecular meshwork. METHODS The anterior segments of normal human eye bank eyes were placed in perfusion organ culture. The effect of various perfusion rates of culture medium, and of the constant flow and constant pressure methods of delivery of cultu...

www.expert-reviews.com ISSN 1746-9899 © 2011 Expert Reviews Ltd 10.1586/EOP.11.70 The retinal pigment epithelium (RPE) is a cellular monolayer between the neural retina and Bruch’s membrane. It adheres to and interacts with the underlying Bruch’s membrane at its basal side and with the photoreceptor outer segments at its apical side. It contributes to a turnover of photoreceptor outer segment, ...

T-cell development requires cytokines and intimate contact with stromal cells provided exclusively by the thymus. Consequently, an in vitro model of thymocyte differentiation, fetal thymic organ culture (FTOC), has been developed. FTOC recapitulates the normal development of T-cells derived from both mouse and human progenitor populations, providing a more rapid means to study T-cell developmen...

The benefit of organ culture is to retain the original structural relationship between various cell species and their interactions and enable us to study the long-term effects of exogenous stimuli. Organ culture methods have been used especially in the studies of the proliferative vascular diseases, such as atherosclerosis and restenosis. We describe here that organ culture is a useful in vitro...

Background: The aim was to evaluate the benefit of technical advances for treatment planning: introduction of a hydrogel spacer, VMAT (volumetric modulated arc therapy) and a single biological organ at risk objective for the rectum and bladder. Initial standard was a step-and-shoot IMRT (intensity modulated radiotherapy) without a spacer and conventional organ at risk objectives.  Material...