Albright hereditary osteodystrophy (AHO) is a disorder caused by GNAS heterozygous inactivation, and results in the spontaneous development of subcutaneous ossifications (SCOs) dermis tissue. We have generated an AHO mouse model through global Gnas inactivation (Gnas+/-) that phenocopies human develops SCOs consistently surround hair follicles (HF). Therefore, this study examined cellular molec...

The bone marrow niche is recognized as a central player in maintaining and regulating the behavior of hematopoietic stem and progenitor cells. Specific gain-of and loss-of function experiments perturbing a range of osteolineage cells or their secreted proteins had been shown to affect stem cell maintenance (Calvi et al, 2003 [1]; Stier et al., 2005 [2]; Zhang et al., 2003 [3]; Nilsson et al., 2...

Correspondence to Stuart A. Aaronson: [email protected] Abbreviations used in this paper: CM, conditioned medium; dnTCF4, dominantnegative TCF4; ERK, extracellular receptor-dependent kinase; HA, hydroxyapatite; hMSC, human MSC; MSC, mesenchymal stem cell; Osx, osterix; PCNA, proliferating cell nuclear antigen; shRNA, short hairpin RNA; TCF, T cell factor; TCP, tri-Ca phosphate. Introduction

Transgenic overexpression of the Notch1 intracellular domain inhibits osteoblast differentiation and causes osteopenia, and inactivation of Notch1 and Notch2 increases bone volume transiently and induces osteoblastic differentiation. However, the biology of Notch is cell-context-dependent, and consequences of Notch activation in cells of the osteoblastic lineage at various stages of differentia...

BACKGROUND The objective of the present study was to evaluate the effect of the ionic product (IP) of BG60S on osteoblastic activity. The following media groups were created: DMEM, which is formed by osteoblasts in basal medium; IP DMEM, which is formed by osteoblasts in IP with basal medium; OST, which is formed by osteoblasts in osteogenic medium; and IP OST, which is formed by osteoblasts in...

Cystine (C)-X-C motif chemokine receptor 4 (CXCR4), the primary receptor for stromal derived factor-1 (SDF-1), is involved in bone morphogenic protein 2 (BMP2)-induced osteogenic differentiation of mesenchymal progenitors. To target the in vivo function of CXCR4 in bone and explore the underlying mechanisms, we conditionally inactivated CXCR4 in osteoprecursors by crossing osterix (Osx)-Cre mic...

Herein is presented a method of classifying hand-drum strokes in real-time by analyzing 50 milliseconds of audio signal as recorded by a contact-mic a xed to the body of the instrument. The classifier performs with an average accuracy of about 95% across several experiments on archetypical strokes, and 89% on uncontrived playing. A complete ANSI C implementation for OSX and Linux is available o...