Background The conventional freezing and vitrification are different cryopreservation protocols for fertility preservation in cancer patients. The high effectiveness of vitrification for human oocytes and embryos is shown, whereas data on human ovarian tissue are limited. Follicular growth, ultrastructure, and apoptosis incidence in human ovarian tissue following vitrification/warming and after...

BACKGROUND Ovarian tissue cryopreservation by vitrification is an attractive technique for fertility preservation in women. However, this technique has not been optimized. The aim of this study was to evaluate the baboon as a model for the preclinical study of ovarian tissue cryopreservation by vitrification and thawing. METHODS Ovarian cortical tissues (1-mm cubes) were obtained surgically f...

Long-term (>7 years) duration of function of ovarian cortical tissue grafts in three patients who have had several successful pregnancies is encouraging and requires us to carefully examine the risk benefit analysis of this technique for our future patients. However, the success rate for ovarian cryopreservation is unclear as the denominator (the number of women in whom frozen-thawed ovarian ti...

Breast cancer is the most frequently occurring cancer in women of developed countries, and as a result of new developments in breast cancer treatment, more women are cured after being diagnosed with this disease. It is important that fertility preservation strategies are addressed before chemotherapy, because chemotherapy may induce premature ovarian failure (depending on the woman's age, the d...

Background The high effectiveness of vitrification, as a cryopreservation protocol, for human oocytes and embryos is shown, whereas data on human ovarian tissue are limited. This study aimed to assessment of follicular growth, ultrastructure, and apoptosis incidence in human ovarian tissue following vitrification/warming and after culture in the presence of LIF. MaterialsAndMethods Ovarian cort...

Cancer treatment modalities are increasingly more effective in achieving complete remission and cure. Aggressive chemotherapy and radiotherapy, as well as bone marrow transplantation, results in >90% cure in many cancers of children and young women. As a result of this success however, a new problem has arisen. Many young women survive to live the rest of their lives in menopause, and have no c...

The preservation of fertility in female cancer survivors has become an important health issue. The different cryopreservation options available for fertility preservation are embryo, oocyte, and ovarian tissue cryopreservation. Oocyte cryopreservation is available for women without partners, but there is a limited experience with this technique and the pregnancy rate is still low. In spite of r...

With the increase in survivorship following cancer for women in their reproductive years, as well as an increase in survivorship with childhood cancers, there is a demand for perfecting current fertility preservation methods and generating new options for patients who are unable to pursue the conventional course of fertility treatments. Cryopreservation using a slow-cooling method for embryos i...

Ovarian cryopreservation followed by transplantation is one of the potential ways fertility can be preserved and endocrine function restored in women who are at risk for ovarian failure, early menopause, or loss of fertility. While much of the progress in this area has occurred in the past couple of decades, the concept of transplantation of reproductive organs has been present since the ninete...

OBJECTIVE To investigate the effect of slow cryopreservation on the morphology and function of primate primordial follicles within ovarian tissue slices. DESIGN Fresh monkey ovarian tissue was frozen by slow cryopreservation and thawed for analysis of morphologic and functional parameters. SETTING University-affiliated laboratory. ANIMALS Rhesus monkey ovarian tissue. INTERVENTION(S) No...