The monarch butterfly (Danaus plexippus) cryptochrome 1 (DpCry1) belongs in the class of photosensitive insect cryptochromes. Here we purified DpCry1 expressed in a bacterial host and obtained the protein with a stoichiometric amount of the flavin cofactor in the two-electron oxidized, FAD(ox), form. Exposure of the purified protein to light converts the FAD(ox) to the FAD*(-) flavin anion radi...

The three-dimensional (3-D) form and the interrelationship of peroxisomes (Po) in the model of regenerating rat liver after partial hepatectomy were studied by computer-assisted 3-D reconstruction of serial ultrathin sections. Po were labeled cytochemically for either catalase, which stains them all uniformly, or for D-amino acid oxidase (DAA-OX), which gives a heterogeneous reaction with light...

To elucidate the reaction mechanism of the disulfide (SS) bond formation reaction of a polypeptide molecule with a water-soluble selenoxide reagent, trans-3,4-dihydroxyselenolane oxide (DHS(ox)), short-term oxidation experiments were carried out for the reduced state (R) of a recombinant hirudin CX-397 variant at pH 7.0 and 25 °C. In the reaction, R was oxidized sequentially to one-SS, two-SS, ...

Reaction of thiodiacetate anion (tda) with [Cu(TMEDA)(NO3)2] (TMEDA = N,N,N'N'-tetramethyl ethylene-diamine) in a stoichiometric ratio afforded the complex {[Cu(TMEDA)(tda)]·(H2TMEDA)(NO3)2·H2O} (1). When [Cu(TMEDA)(NO3)2] was added in excess, it yielded a μ-oxalato complex {[Cu2(TMEDA)2(μ-ox)(H2O)2](NO3)2·2CH3CN} (2). The reaction involves a facile room temperature desulfurization of the thiod...

Abstract The stoichiometry of ozone and hypochlorous acid reactions with lignin hexenuronic (HexA) was measured in bleaching experiments Eucalyptus sp. kraft pulp. progress the followed by UV Resonance Raman spectroscopy that can quantify HexA based on scattering intensities carbon–carbon double bond aromatic ring lignin. Here, one mol converted 0.16 (C9 monomer units) 0.28 HexA, whereas 1 0.09...

oxidized ldl (ox-ldl) lipoproteins are proposed as important modified particles triggering pro-inflammatory events through receptor-mediated pathways. we evaluated the circulating ox-ldl level on the concept that the chronic immune events may affect ox-ldl clearance as the vessel stenosis develops in coronary arteries. one hundred sixty five subjects underwent coronary angiography and then, sub...

In recent years, the concentrations of PM2.5 in urban ambient air China have been declining; however, strong atmospheric oxidation capacity (AOC) represents challenges to further reduction concentration and continuous improvement quality future, since overall AOC is still at a high level. For this paper, based on ground observation data recorded Beijing from 2016 2019, variation was characteriz...

Objective: In patients with diabetes mellitus, increased rate of lipoprotein oxidation and oxidative stress have important role in diabetic angiopathy, including nephropathy. We designed a study for comparing the level of Ox-LDL in diabetic patients with Macro-albuminuria, Micro-albuminuria, versus Normo-albuminuric. Materials and Methods: One hundred and thirty five patients with type 2 dia...

Zinc-air batteries have several advantages in comparison with the lithium-ion technology as they enable use of earth-abundant elements, work at low cost, are lightweight, and also much safer application. In addition to chemistry related zinc electrode, efficient stable bifunctional catalysts required for oxygen reduction reaction (ORR, discharging) evolution (OER, charging) on air-electrode sid...