Males develop kidney stones far more frequently than females with a ratio of 2-3:1, suggesting that androgen receptor (AR) signaling might play a key role in the development of nephrolithiasis. Using the cre-loxP system to selectively knock out AR in glyoxylate-induced calcium oxalate (CaOx) crystal mouse models, we found that the mice lacking hepatic AR had less oxalate biosynthesis, which mig...

An amperometric flow biosensor for oxalate determination in urine samples after enzymatic reaction with oxalate oxidase immobilized on a modified magnetic solid is described. The solid was magnetically retained on the electrode surface of an electrode modified with Fe (III)-tris-(2-thiopyridone) borate placed into a sequential injection system preceding the amperometric detector. The variables ...

BACKGROUND Although autistic spectrum disorders (ASD) are a strongly genetic condition certain metabolic disturbances may contribute to clinical features. Metabolism of oxalate in children with ASD has not yet been studied. AIM The objective was to determine oxalate levels in plasma and urine in autistic children in relation to other urinary parameters. METHOD In this cross-sectional study,...

Urinary oxalate plays an important role in the formation of calcium oxalate stone, and endogenous oxalate metabolism mainly occurs in the liver. Since dehydroepiandrosterone (DHEA) is known to have an effect on hepatocellular proliferation and on some hepatic enzymes, we examined the influence of DHEA on the activity of hepatic oxalate-related enzymes and on urinary oxalate excretion in rats. F...

ABSTRACT Sclerotinia sclerotiorum is a necrotrophic pathogen that devastates the yields of numerous crop species, including beans. The disease in common bean and pea is referred to as white mold. We examined the relationship between oxalate, an established virulence factor of S. sclerotiorum, and partial white mold resistance of scarlet runner bean (Phaseolus coccineus). P. coccineus genotypes ...

BACKGROUND Exposure of renal epithelial cells to oxalate (Ox) or calcium oxalate (CaOx) crystals leads to the production of reactive oxygen species and cell injury. We have hypothesized that Ox and CaOx crystals activate NADPH oxidase through upregulation of its various subunits. METHODS Human renal epithelial-derived cell line, HK-2, was exposed to 100 μmol Ox or 66.7 μg/cm(2) CaOx monohydra...

This work describes a novel method of urinary oxalate determination based on a catalase model compound MnL(H2O)2(ClO4)2 (L 1⁄4 bis(2-pyridylmethyl)amino)propionic acid). Urinary oxalate is first decomposed by the oxalate oxidase into carbon dioxide and hydrogen peroxide. The later is then disproportionated into water and oxygen by the catalase model compound forming a color compound, which can ...

BACKGROUND Though resveratrol is known to have anti-cancer, anti-diabetic, anti-oxidant and anti-inflammatory activities, the inhibitory mechanism of resveratrol in kidney stone formation has not been elucidated so far. METHOD ELISA, flow cytometry, RT-PCR, and western blotting were performed. Human renal epithelial cells (HRCs) and rats with ethylene glycol (EG)-induced kidney stones were us...

Molecular investigations during wheat germination have revealed unique developmentally regulated proteins, designated as germins, which show remarkable resistance to broad specificity proteases and to dissociation in SDS. Germins in cereals have an oxalate oxidase activity, which generates H2O2 from the oxidative breakdown of oxalate thereby playing a significant role in plant development and d...