AIM The status of vaginal lacto-bacillary flora, an indicator of possible genital infection and pregnancy complications, can be assessed on wet mount or Gram stained specimens. The former is quick, the latter more routine. The accuracy of the two preparative techniques to detect normal vaginal lacto-bacillary microflora was compared for 646 patients. The effect of delay in transport medium befo...

Bartholomew and Mittwer (1951) presented photographic evidence that the gram-positive staining area of bacterial cells could be fitted internally to the area demonstrated by the Dyar cell wall stain. Their conclusion was that the bacterial cell wall was not included in the grampositive area. Lamanna and Mallette (1954) explained Gram differentiation of intact cells on the basis that crystal vio...

Products of thiocyanate oxidation by lactoperoxidase inhibit gram-positive bacteria that produce peroxide. We found these products to be bactericidal for such gram-negative bacteria as Pseudomonas species and Escherichia coli, provided peroxide is supplied exogenously by glucose oxidase and glucose. By the use of immobilized glucose oxidase the bacterial agent was shown to be dialyzable, destro...

conclusions concurrent pulmonary nocardiosis and tuberculosis is frequent in hiv-infected patients. rapid and sensitive methods such as pcr are recommended for detection of such co-infections. results from 157 sputum specimens, positive samples by acid fast staining, culture and pcr for m. tuberculosis were reported for 7.6% (12/157), 10.1% (16/157) and 7% (11/157) of samples, respectively. no ...

Microorganisms have the ability to adapt most varied environments, they are potential sources of infection, inanimate surfaces in places great circulation people, enable transmission these infectious agents capable colonizing and infecting an individual main route microorganisms occurs through hands. The objective study was isolation identification bacteria on religious tourism spots Juazeiro d...

A 21-year-old man presented with a painless ulcer on the penis, which had been present for 4 weeks, and diffuse, symmetrical erythematous maculopapules on the trunk, extremities, palms, and soles, which had been present for 1 week. Gram staining of bloody exudate from his chancre revealed very weakly Gram-negative spiral rods (Picture 1; × 1,000). We therefore performed Giemsa staining, which r...

In the present study, strain MHDS3 was isolated from a water sample of Çamaltı Saltern and identified using conventional molecular methods. 16S rRNA gene sequence analyses showed that belonged to Planococcus dechangensis species. It gave positive result in Gram staining test. The cells were coccus, non-motile, aerobic, catalase positive, oxidase negative colony pigmentation yellow-orange. resul...

The search for alternative methods treating and preventing bee dysbacteriosis is a priority beekeeping as branch of veterinary medicine. Lime honey, buckwheat flower forest acacia honey were tested to evaluate their antagonistic effect against test culture enterobacteria bees Klebsiella pneumoniae species. study was conducted in several stages: 1. Determine the activity microflora pure pathogen...