Activity of the adeno-associated virus P5 transcriptional control region was found to be induced by adenovirus E1A gene products. A pair of adjacent sequence elements was found to mediate both basal and E1A-induced P5 activity. The first element is a binding site for the major late transcription factor (MLTF), a factor first identified on the basis of its binding to a specific sequence within t...

The complexity status of the maximum stable set problem in the class of P5-free graphs is unknown. In this paper, we 3rst propose a characterization of all connected P5-free augmenting graphs. We then use this characterization to detect families of subclasses of P5-free graphs where the maximum stable set problem has a polynomial time solution. These families extend several previously studied c...

Our special fuzzy classifier operates on the set of eight features extracted from the 3x3 neighborhood of each pixel. These features are the magnitudes of differences between that pixel and the eight neighboring pixels. They are input into the fuzzy classifier inputs that connect to two fuzzy set membership functions that represent “white background” or “black edge.” The paradigm is simple, com...

The p5 promoter region of adeno-associated virus type 2 (AAV-2) is a multifunctional element involved in rep gene expression, Rep-dependent replication, and site-specific integration. We initially characterized a 350-bp p5 region by its ability to behave like a cis-acting replication element in the presence of Rep proteins and adenoviral factors. The objective of this study was to define the mi...

The Pancasila Student Profile Strengthening Project, or P5, is a cross-discipline project based on problems in the education sector. Since 2021/2022 academic year, there have been 4 themes for early childhood (Pendidikan Anak Usia Dini PAUD), and 7 elementary schools (Sekolah Dasar SD), middle Menengah Pertama SMP), high Atas SMA). P5 makes up around 20-30% of total credits year. However, teach...

BACKGROUND Polybasic helical peptides, such as peptide p5, bind human amyloid extracts and synthetic amyloid fibrils. When radiolabeled, peptide p5 has been shown to specifically bind amyloid in vivo thereby allowing imaging of the disease. Structural requirements for heparin and amyloid binding have been studied using analogs of p5 that modify helicity and chirality. METHODS Peptide-ligand i...

Francisella tularensis subspecies tularensis, the etiological agent of tularemia, is highly pathogenic to humans and animals. However, the SCHU strain of F. tularensis SCHU P0 maintained by passaging in artificial media has been found to be attenuated. To better understand the molecular mechanisms behind the pathogenicity of F. tularensis SCHU, we attempted to isolate virulent bacteria by seria...

To investigate the relative importance of each of the ribose 3'-hydroxyl groups of 2-5A (ppp5' A2'p5'A2'-p5' A) in determining binding to and activation of the 2-5A-dependent endonuclease (RNase L), the 3'-hydroxyl functionality of each adenosine moiety of 2-5A trimer triphosphate was sequentially replaced by hydrogen. The analog in which the 5'-terminal adenosine was replaced by 3'-deoxyadenos...

Let G1, G2, . . . , Gt be graphs. The multicolor Ramsey number R(G1, G2, . . . , Gt) is the smallest positive integer n such that if the edges of a complete graph Kn are partitioned into t disjoint color classes giving t graphs H1,H2, . . . ,Ht, then at least one Hi has a subgraph isomorphic to Gi. In this paper, we provide the exact value of R(Pn1 , Pn2 , . . . , Pnt , Ck) for certain values o...