A molecular survey was performed for identification Theileria spp in sheep and ticks during from 2010-2011 in Fasa and Kazeroun areas, Fars province, Iran. A total of 100 sheep from different flocks were clinically examined and blood samples with ixodid ticks collected. The prepared blood smears from capillary vein of ear were stained with giemsa methods and examined by using light microscope. ...

While real-time-polymerase chain reaction (RT PCR) has been used as a rapid test for detection of Salmonella Enteritidis in recent years, little research has been done to assess the feasibility of pooling poultry environmental samples with a Salmonella Enteritidis-specific RT PCR assay. Therefore the objective of this study was to compare RT PCR Salmonella Enteritidis detection in individual an...

a molecular survey was performed for identification theileria spp in sheep and ticks during from 2010-2011 in fasa and kazeroun areas, fars province, iran. a total of 100 sheep from different flocks were clinically examined and blood samples with ixodid ticks collected. the prepared blood smears from capillary vein of ear were stained with giemsa methods and examined by using light microscope. ...

Flea-borne pathogens were screened from 100 individual cat fleas using a PCR approach, of which 38 % infected with at least one bacterium. Overall, 28 the flea samples positive for Bartonella as inferred ITS DNA region. Of these, 25 (7/28) identified clarridgeiae, 42.9 (12/28) henselae consisted two different strains, and 32.1 (9/28) koehlerae, was detected first time in Malaysia. Sequencing gl...

this study was conducted with the aim of determining surface water contamination with cysts of entamoeba histolytica using pcr in rasht city, northern iran.in this cross-sectional study, 49 water samples including 18 rivers and 6 wetlands were collected from different regions near the city of rasht in autumn of 2012. after filtration using 0.22 μm nitrate cellulose membrane filters, the samples...

Fecal samples were examined for viruses participated in gastrointestinal disorders of cats, especially focusing on feEine coronavirus <FCoV) by a reverse transcriptase-polymerase chain reaction assay. It was found that a primary viral pathegen was feline panleukopenia parvovirus (FPLV; 28.5% of the positive rate) and the secondary was FCoV "O.7%). Comrnonly reported clinical signs of cats of wh...

Tuberculosis (TB) is a chronic infectious disease that has been threatening public health for many centuries. The clinical diagnostic procedure for TB is time-consuming and laborious. In the last 20 years, real-time fluorescence-based quantitative PCR (real-time PCR) has become a better alternative for TB diagnosis in clinics due to its sensitivity and specificity. Recently, digital droplet PCR...