Ultraviolet (UV)-induced cataracts are becoming a major environmental health concern because of the possible decrease in the stratospheric ozone layer. Experiments were designed to isolate gene(s) affected by UV irradiation in rabbit cornea tissues using fluorescent differential display-reverse transcription-polymerase chain reaction (FDDRT-PCR). The epithelial cells were grown in standard medi...

Of the various synthetic biodegradable polymers, polylactide, poly(butylene succinate), and poly(hydroxyalkanoate) have been widely studied developed. This review focuses on studies of chain end modification these polymers. The selected research was categorized into initiators, modification, including extending, degradation reaction. We cover recent literature this topic, discuss developing tre...

giardia is one of the most common human parasites and causes a lengthly course of nonbacterial diarrhea. disease outbreaks due to giardia infection are often attributed to contaminated water supplies. a major problem associated with detection for this organism is the lack of sensitive and reliable methods. pcr has the potential to address many of the limitations.we have performed a pcr-based me...

background and aims: influenza virus is a major pathogen involved in respiratory illnesses during winter seasons. a variety of diagnostic methods have been developed to identify influenza viruses in clinical specimen. methods: nasal and pharyngeal samples taken from patients were inoculated into madin-darby canine kidney (mock) cells and embryonated chicken eggs (eces). the culture media was as...

background and aims: influenza a virus infects birds and some mammalian including human. h1 and h3 subtypes are circulated in both human and birds’ population. to determine the prevalence of the mentioned subtypes in birds, different captive bird species in tehran zoo, and affiliated centers in tehran were investigated for virus infection. methods: in this study, between november 2008 and febru...

background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...

abstract: introduction & objective: helicobacter pylori are among the important pathogens responsible for chronic gastritis, peptic ulcer and gastric cancer. the present study aimed to comparatively evaluate pcr and rut methods for detection of h. pylori and determination of the prevalence of the cagapositive helicobacter pylori strains in shahrekord. materials & methods: this is a molecular ep...