Pectins are fundamental polysaccharides in the plant primary cell wall. Pectins are synthesized and secreted to cell walls as highly methyl-esterified polymers and then demethyl-esterified by pectin methylesterases (PMEs), which are spatially regulated by pectin methylesterase inhibitors (PMEIs). Although PME and PMEI genes are pivotal in plant cell wall formation, few studies have focused on t...

Knowledge of how proteins and polysaccharides interact is the key to understanding encapsulation and emulsification in these composite systems and ultimately to understanding the structures of many biological network systems. As a model system we have studied β-lactoglobulin A (βLgA) interacting with pectins of various amounts and distribution patterns of charge. The studies were conducted at p...

Comprehensive chemical characterization of nine mono-varietal apple pomaces obtained from the production ciders with PDO is described. They were rich in essential minerals, fibers (35–52.9 %), and polyphenols. High levels GalA (11.8–21.6 revealed suitability these as efficient sources pectins. Extracted pectins showed high variability monomer composition, degrees methylesterification, strongly ...

Pectins analysed by AFM are visualized as individual chains, branched or unbranched, and aggregates. To investigate the nature of these structures, sodium carbonate soluble pectins from strawberry fruits were digested with endo-Polygalacturonase M2 from Aspergillus aculeatus and visualized by AFM. A gradual decrease in the length of chains was observed as result of the treatment, reaching a min...

A chemometric study was carried out in order to typify cider apples according to their degree of ripening. Several chemical variables (sugars, organic acids, amino acids, polyphenols, and pectins) were analyzed using HPLC and FIA methods. Univariate data treatment was not sufficient to allow the apple varieties to be differentiated according to their stage of ripening. Two linear combinations o...

Manipulating and stretching single pectin molecules offer convenience to determine the chain lengths and further illustrate the action between pectins and other macromolecules. Pectin molecules on mica surfaces, which were imaged in air with atomic force microscopy (AFM), can be aligned and stretched through applying modified molecular combing and fluid fixation techniques. Modified molecular c...

The effects on blood and faecal lipids of the addition of 15 g of either high- or low-methoxyl pectin to the habitual diets of ten healthy young adults have been studied. 2. Serum total cholesterol levels were reduced by a mean of 16% during consumption of low-methoxyl pectin and 18% during consumption of high-methoxyl pectin. High density lipoprotein cholesterol levels and triglyceride levels ...

The methyl esters of carboxylic acids are characteristic olfactory volatile compounds for the sweet aroma of snake fruit, (Salacca edulis, Reinw) cv. Pondoh. Although methanol was not detected as a volatile constituent, the crude enzymes showed activity to synthesize the methyl esters in the presence of acyl-CoA and methanol. Therefore, the biosynthetic origin of methanol was investigated, resu...