Sickle cell disease (SCD) is a severe genetic hemoglobin disorder that results in premature destruction of red blood cells. Assessment the severity challenging task clinical routine, since causes broad variance SCD manifestation despite common cause remain unclear. Identification biomarkers would predict grade importance for prognosis and assessment responsiveness patients to therapy. Detection...

Chromaffin vesieles were first purified by differential and density gradient centrifugation in isotonie (Percoll) gradients. In subsequent suerose gradients p38jsynaptophysin exhibited the same distribution as established marker substances of chromaffin vesieles. Quantification of immunoblots revealed that 750 ng p38{synaptophysin per mg of protein were present in the chromaffin vesieles reeove...

1. Synaptosomes Kiebler et al [1] was the first group to describe the use of a discontinuous iodixanol gradient in the isolation of dendritic spines from mouse hippocampus. The gradient comprised four layers of 9%, 12.5%, 15% and 25% (w/v) iodixanol (equivalent to densities of 1.076, 1.095, 1.105 and 1.152 g/ml); the densest solution contained the material pelleted from the hippocampal homogena...

Medical assistance for procreation in a couple where one or both parents has hepatitis C viral infection (HCV) raises the issue of the transmission of the infection to the baby and/or of possible contamination of both the technicians and the gametes or embryos from virus-free parents in the laboratory. It becomes essential to assess transmission risk in assisted reproductive techniques in order...

OBJECTIVE In assisted reproduction, spermatozoa must be effectively separated from seminal plasma to undergo capacitation, a prerequisite for fertilization. Percoll was recently withdrawn from the American market because of safety concerns. The purpose of this study was to evaluate the quality of sperm separated by two different sperm washing media, and compare these results to sperm quality af...

To evaluate and optimize the sperm separation efficiency of a novel silane-coated silica bead (Puresperm), serial studies were carried out to compare the various sperm parameters between: (i) three-layer (90%-70%-40%) Puresperm and three-layer (90%-70%-40%) conventional polyvinylpyrrolidone (PVP)-coated silica bead (Percoll) gradients; (ii) three-layer (90%-70%-40%) and two-layer (90%-45%) Pure...

Sperm cells were isolated from corn (Zea mays L.) tricellular pollen grains. They were released using a light osmotic chock, and separated from pollen contaminants (especially starch grains) by a Percoll gradient centrifugation. Isolated sperm cells (3 x 10(6) per milliliter) show a high viability score (90%) as demonstrated with the fluorochromatic reaction. They appeared as spherical cells wh...

Killer lymphocytes induce apoptosis by the release of cytotoxic mediators from specialized secretory lysosomes, called cytotoxic granules, into the immunological synapse formed with a cell targeted for elimination. Methods are presented here for isolating CTL and NK cell cytotoxic granules using cell disruption by nitrogen cavitation followed by continuous Percoll density gradient fractionation...

Crude chromaffin secretory vesicles, obtained by differential centrifugation, were further purified on isotonic (Percoll) gradients. The chromaffin vesicle fractions recovered from the gradients contain acetylcholinesterase as well as lysosomal enzymes. With the aid of a subsequent sucrose gradient lysosomal enzymes could be removed from chromaffin vesicle fractions, but not acetylcholinesteras...