White-rot fungi (WRF) and their ligninolytic enzymes (laccases and peroxidases) are considered promising biotechnological tools to remove lignin related Persistent Organic Pollutants from industrial wastewaters and contaminated ecosystems. A high diversity of the genus Ganoderma has been reported in Cuba; in spite of this, the diversity of ligninolytic enzymes and their genes remained unexplore...

Oxidative stress is a disturbance in the equilibrium among free radicals, reactive oxygen species, and endogenous antioxidant defense mechanisms. Oxidative stress is a result of imbalance between the production of reactive oxygen and the biological system's ability to detoxify the reactive intermediates or to repair the resulting damage. Mounting evidence has implicated oxidative stress in vari...

Plant peroxidases are capable of binding phenolic substrates, and it has been possible to crystallize complexes between horseradish peroxidase C (HRP C) and benzhydroxamic acid. The X-ray structures of the binary HRP C:ferulic acid complex and the ternary HRP C:CN(-):ferulic acid complex to 2.0 and 1.45 A resolution, respectively, have also been solved recently. Ferulic acid is a naturally occu...

White rot fungi use a variety of mechanisms to accomplish the complete degradation of lignin and a wide variety of environmental pollutants. Both oxidative and reductive reactions are required for the metabolism of both lignin and environmental pollutants. The fungi secrete a family of peroxidases to catalyze both direct and indirect oxidation of chemicals. The peroxidases can also catalyze red...

Oxidative decarbyxylation of p-hydroxybenzoic acids in plant cell suspension cultures is catalyzed by peroxidases. This reaction has been characterized in vivo and in vitro. Decarboxylation of substituted benzoic acids yields monomeric, dimeric and oligomeric benzoquinones. All peroxidases obtained from soybean (Glycine max) cell suspension cultures by gel electrophoresis are equally capable to...

A challenge in the redox field is the elucidation of the molecular mechanisms, by which H2O2 mediates signal transduction in cells. This is relevant since redox pathways are disturbed in some pathologies. The transcription factor OxyR is the H2O2 sensor in bacteria, whereas Cys-based peroxidases are involved in the perception of this oxidant in eukaryotic cells. Three possible mechanisms may be...

Peroxidases are haem-containing enzymes which catalyse the reduction of hydrogen peroxide by different organic substrates. Plant, fungal and bacterial peroxidases are evolutionarily related and constitute the plant peroxidase superfamily, which can be divided into three structural classes. Class I, of prokaryotic origin, includes cytochrome c peroxidase (CCP); class I1 contains secretory fungal...

A cDNA library was constructed from leaf epidermis of diploid wheat (Triticum monococcum) infected with the powdery mildew fungus (Blumeria graminis f. sp. tritici) and was screened for genes encoding peroxidases. From 2,500 expressed sequence tags (ESTs), 36 cDNAs representing 10 peroxidase genes (designated TmPRX1 to TmPRX10) were isolated and further characterized. Alignment of the deduced a...

Abstract The vascular wilting caused by Fusarium oxysporum f. sp. dianthi (Fod) is the most relevant disease for carnation cultivation. Understanding biochemical mechanisms involved in resistance to Fod will allow development of new control strategies. In this research, levels some phytohormones such as salicylic acid (SA), methyl salicylate (MeSA), and jasmonate (MeJA) were evaluated symplast ...