Abstract Purpose To build a more holistic understanding of soil pH change we assessed the synchronised effects contrived on chemistry, vegetation growth and nutrition, faunal abundance diversity. Methods We established fifteen year old field experiment with gradient (pH 4.3 to 6.3) measured effect plant biomass elemental composition impact these changes fauna (earthworms, nematodes, rotifers ta...

The relationship between the electrochemical proton gradient (delta mu-H+) and the electrochemical gradient for biogenic amines (delta mu-A) was investigated in isolated chromaffin ghosts free of endogenous components and gradients. The addition of ATP to a ghost suspension resulted in the generation of a large proton concentration gradient (delta pH), acidic inside (measured by [14C]-methylami...

We report on the preparation and characterization of thin polyampholytic hydrogel gradient films permitting pH-controlled protein resistance via the regulation of surface charges. The hydrogel gradients are composed of cationic poly(2-aminoethyl methacrylate hydrochloride) (PAEMA), and anionic poly(2-carboxyethyl acrylate) (PCEA) layers, which are fabricated by self-initiated photografting and ...

Background A transpulmonary gradient (TPG)>12mmHg is thought to represent evidence of vascular change beyond that expected from passive pulmonary venous congestion in patients with pulmonary hypertension and left heart disease (PH-LHD). However recent studies found those with a diastolic pressure gradient (DPG) >6 to have a worse survival. This has led to a change in the recent guidelines sugge...

Two-dimensional (2-D) electrophoresis as it was introduced by O’Farrell in 1975 [ 11 is unsurpassed by any other technique for simultaneously resolving hundreds of polypeptides. The usefulness of this method has been documented in thousands of publications. However, 2-D electrophoresis is still a demanding procedure with respect to performance and required expertise [2-41. Because of the large ...

A vacuolar H+-ATPase-negative mutant of Saccharomyces cerevisiae was highly sensitive to nickel ion. Accumulation of nickel ion in the cells of this mutant of less than 60% of the value for the parent strain arrested growth, suggesting a role for this ATPase in sequestering nickel ion into vacuoles. An artificially imposed pH gradient (interior acid) induced transient nickel ion uptake by vacuo...