نتایج جستجو برای: phage library

تعداد نتایج: 138241  

Journal: :reports of biochemistry and molecular biology 0
farideh hosseinzadeh shiraz hiv/aids research center, shiraz university of medical sciences, shiraz, iran-recombinant antibody laboratory, department of immunology, shiraz university of medical sciences, shiraz, iran s. saeed mohammadi shiraz hiv/aids research center, shiraz university of medical sciences, shiraz, iran-recombinant antibody laboratory, department of immunology, shiraz university of medical sciences, shiraz, iran foroogh nejatollahi tel: +98 71 32351575; fax: +98 71 32351575

background:  cytotoxic t lymphocyte–associated antigen 4 (ctla-4) molecules are expressed on t-cells and inhibit their function by inhibiting activation of subsequent t-cell molecular pathways. blocking of ctla-4 inhibits the growth of malignant tumor cells. anti-ctla-4 monoclonal antibodies activate the immune system against cancer. due to several advantages of single-chain antibodies (scfvs) ...

2009
Emmanuel Dias-Neto Diana N. Nunes Ricardo J. Giordano Jessica Sun Gregory H. Botz Kuan Yang João C. Setubal Renata Pasqualini Wadih Arap

BACKGROUND Combinatorial phage display has been used in the last 20 years in the identification of protein-ligands and protein-protein interactions, uncovering relevant molecular recognition events. Rate-limiting steps of combinatorial phage display library selection are (i) the counting of transducing units and (ii) the sequencing of the encoded displayed ligands. Here, we adapted emerging gen...

Journal: :Journal of controlled release : official journal of the Controlled Release Society 2008
Chunxiao Wu Seong Loong Lo Jerome Boulaire Michelle Li Wen Hong Hui Min Beh Doreen Siu Yi Leung Shu Wang

Aiming at identification of novel peptides that can be employed for effective targeting of malignant gliomas, we used a 12-mer peptide phage display library and cultured human malignant glioma cells for phage selection. Several common phage clones emerged after 4 rounds of biopanning against the U87MG glioblastoma cell line. The most abundant phage clone VTW, expressing a sequence of VTWTPQAWFQ...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2002
Bin Zhou Peter Wirsching Kim D Janda

A naive, human single-chain Fv (scFv) phage-display library was used in bio-panning against live, native spores of Bacillus subtilis IFO 3336 suspended in solution. A direct in vitro panning and enzyme-linked immunosorbent assay-based selection afforded a panel of nine scFv-phage clones of which two, 5B and 7E, were chosen for further study. These two clones differed in their relative specifici...

Journal: :Angewandte Chemie International Edition 2019

Journal: :"Protein Engineering, Design and Selection" 1996

Journal: :Monoclonal antibodies in immunodiagnosis and immunotherapy 2014
Junko Muraoka Takuya Ozawa Yurie Enomoto Norihiko Kiyose Ayana Imamura Kazunari Arima Hiroshi Nakayama Yuji Ito

A new single-chain variable fragment (scFv) antibody library was generated and human serum albumin (HSA)-specific clones were characterized to investigate the usefulness of porcine antibodies. Phage libraries were developed from pigs immunized with the model antigen HSA. The library size was 1.5 × 10(7) for kappa (VL) and 1.4 × 10(7) for lambda fragments. Eight HSA-specific clones from the kapp...

Journal: :Angewandte Chemie 2010
Ratmir Derda Sindy K Y Tang George M Whitesides

Uniform amplification of a mixture of phage clones is central to the selection of peptides and proteins presented on the coat proteins of phage (phage display). Uniform amplification cannot be achieved when phage having different rates of growth compete with each other in a common solution. Here we describe a method for uniform amplification of individual phage clones, from a mixture of clones ...

Journal: :Protein engineering 1996
V A Petrenko G P Smith X Gong T Quinn

A billion-clone library of filamentous phage with different surface structures ("landscapes') was generated by fusing random octapeptides to the N-terminus of all 4000 copies of the major coat protein. Such a "landscape library' might include clones exhibiting emergent properties that inhere in the entire surface architecture, not in the peptides by themselves. Because the diverse surface lands...

Journal: :Journal of immunological methods 2002
Naoki Shinohara Hiroo Fukuda

A combination of phage display antibody technology and a subtraction method provides a powerful tool for the isolation of novel biomarkers. However, the dilemma that high stringency screening often reduces the diversity in the subtracted phage antibody repertoires and that it is difficult to isolate phage antibody against rare epitopes remain. Therefore, we carefully monitored the occupancy of ...

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