Aqueous two-phase system (ATPS) is considered a new method to remove heat stable salts (HSS) from flue gas desulfurizer 1,4-bis-(2-hydroxyethyl)-piperazine (BHEP). In this work, the liquid-liquid phase equilibrium data of BHEP-Na2SO4 ATPS in 303.15 – 323.15 K were measured and correlated by NRTL equation with redefined adjustable characteristic constant at different concentrations. It revealed ...

Surface properties of Streptomyces griseus cells, such as surface net charge and surface net hydrophobicity, were characterized by using the aqueous two-phase system (ATPS) method. It was found that the surface net charge of S. griseus cells was reduced after the POPC liposome-treatment of the cells at 41C. The surface net hydrophobicities of S. griseus cells and liposome-treated cells cultivat...

Compartmentalization in a prebiotic setting is an important aspect of early cell formation and is crucial for the development of an artificial protocell system that effectively couples genotype and phenotype. Aqueous two-phase systems (ATPSs) and complex coacervates are phase separation phenomena that lead to the selective partitioning of biomolecules and have recently been proposed as membrane...

This study aimed to purify and partially characterize a keratinolytic protease produced by Bacillus sp. P45 through bioconversion of feather meal. Crude extract was purified using sequence an aqueous two-phase system (ATPS) in large volume systems (10, 50, 500 g) increase obtaining enzyme, followed diafiltration (DF) step. Purified characterized terms protein profile analysis SDS-PAGE, optimum ...

The polysaccharide maltodextrin (Mdx) can provide a low cost alternative to substitute the fractioned dextran for the use with polyethylene glycol (PEG) in aqueous two-phase polymer polymers systems. Aqueous two-phase system (ATPS) partitioning has been used to recover and concentrate proteins from Shrimp Waste and offers many advantages along with biomass removal. In this work we have studied ...

In this work, a two-step extraction methodology of ionic liquid-based ultrasonic-assisted extraction (IL-UAE) and ionic liquid-based aqueous two-phase system (IL-ATPS) was developed for the extraction and purification of secoisolariciresinol diglucoside (SDG) from flaxseed. In the IL-UAE step, several kinds of ILs were investigated as the extractants, to identify the IL that affords the optimum...

Monoclonal antibodies have proven their potential as therapeutic molecules with applications as diverse as diagnosis, oncology and treatment of autoimmune diseases; the need for their supply is higher than it ever was, with the global market for monoclonal antibodies being expected to grow to nearly $58 billion in 2016. The main challenge for this growth remains the fact these products need to ...

This paper presents a method to form micron-sized droplets in an aqueous two-phase system (ATPS) and to subsequently polymerize the droplets to produce hydrogel beads. Owing to the low interfacial tension in ATPS, droplets do not easily form spontaneously. We enforce the formation of drops by perturbing an otherwise stable jet that forms at the junction where the two aqueous streams meet. This ...

1 Abstract Researchers of reasoning in computer science and psychology are estranged sib-lings. The tools they use to investigate patterns of inference seldom overlap, because the goals of each group differ. A computer scientist's primary goal is to efficiently engineer systems based on logical calculi. But, human reasoning systematically violates the constraints imposed by orthodox logic. One ...

PURPOSE Awaiting trial prisoners (ATPs) are represented in prisons globally, and may stay for long periods in detention. This group is however underrepresented in literature on incarcerated persons. We aim to explore the lived experiences of ATPs detained for prolonged years in a sub-Saharan country; examining what they make of their status and how their conditions have affected their wellbeing...