Poplar (Populus tremula x alba) trees (clone INRA 717-1-B4) were cultivated for 1 month in phytotronic chambers with two different levels of ozone (60 and 120 nL L(-1)). Foliar activities of shikimate dehydrogenase (EC 1.1.1.25), phenylalanine ammonia lyase (EC 4.3.1.5), and cinnamyl alcohol dehydrogenase (CAD, EC 1.1.1.195) were compared with control levels. In addition, we examined lignin con...

The present study was conducted to investigate the allelopathic activity of Rumex dentatus aqueous leaf extract on metabolites, reactive oxygen species (ROS), and enzymes activities in Portulaca oleraceae. results showed a reduction carbohydrates, protein, DNA, RNA contents. However, total phenolics, flavonoids, malondialdehyde were enhanced. ROS such as hydrogen peroxide, superoxide radicals, ...

The molecular structures of recombinant L-phenylalanine dehydrogenase from Rhodococcus sp. M4 in two different inhibitory ternary complexes have been determined by X-ray crystallographic analyses to high resolution. Both structures show that L-phenylalanine dehydrogenase is a homodimeric enzyme with each monomer composed of distinct globular N- and C-terminal domains separated by a deep cleft c...

Several derivatives of phenylalanine and tyrosine were prepared and tested for inhibition of chorismate mutase-prephenate dehydrogenase (EC 1.3.1.12) from Escherichia coli K12 (strain JP 232). The best inhibitors were N-toluene-p-sulphonyl-L-phenylalanine, N-benzenesulphonyl-L-phenylalanine and N-benzloxycarbonyl-L-phenylalanine. Consequently two compounds, N-toluene-sulphonyl-L-p-aminophenylal...

Pinus thunbergii (P. thunbergii) is a gymnosperm with important economic and ecological value. In order to investigate the diagnosis defense mechanism of P. against Bursaphelenchus xylophilus (the pinewood nematode, PWN), needles seedlings on fifth day after being infected by PWN were taken as samples for transcriptome sequencing analysis. Compared control group, 647 genes differentially expres...

Bienzymatic amperometric biosensor consisting of L-phenylalanine dehydrogenase and diaphorase for the determination of L-phenylalanine has been developed. Enzymes were immobilized between chitosan layers onto the surface of planar nanocomposite electrodes based on multi-walled carbon nanotubes. Wide linear concentration range was obtained for 12–1250 μM of L-phenylalanine in phosphate buffer so...

The tryptic peptide containing the active-site cysteine in glyceraldehyde-3-phosphate dehydrogenase from Bacillus stearothermophilus 1503 was isolated after inhibition of the enzyme with (14)C-iodoacetate. The amino acid sequence of the 20-residue peptide was determined by 19 successive cycles of dansyl-Edman degradation. The sequence shows considerable homology with its counterparts from mesop...

A new NAD+-dependent opine dehydrogenase was purified to homogeneity from Arthrobacter sp. strain 1C isolated from soil by an enrichment culture technique. The enzyme has a molecular weight of about 70,000 and consists of two identical subunits with molecular weights of about 36,000. The enzyme catalyzed a reversible oxidation-reduction reaction of opine-type secondary amine dicarboxylic acids....