The separation of myo-inositol phosphates by ion pair, reverse-phase high performance liquid chromatography has been investigated. The retention of the inositol phosphates is dependent on both the polarity of the hetaeron utilized and on the pH of the solvent. A method is presented which permits the isocratic separation of multiple forms of inositol phosphates including isomers of myo-inositol ...

Heats of immersion of alkali and alkaline earth metaphosphates in some aromatic hydrocarbons were measured with a twin microcalorimeter to investigate the interaction of the interfaces between the phosphates and the hydrocarbons. The heats of immersion in benzene for the phosphates with long chains were lower than those for the phosphates with short chains. The dependence of heat of immersion o...

The accessibility of phosphates towards ethylnitrosourea was studied in tRNA/aminoacyl-.tRNA synthetase complexes and in the ternary aminoacyltRNA/EF-Tu/GTP complex. When the tRNA is complexed to aminoacyl-tRNA synthetases, several phosphates are protected against the alkylation agent, the protection patterns varying according to the tRNA/aminoacyl-tRNA synthetase system. However in all cases p...

The central role that phosphates play in biological systems, suggests they also played an important role in the emergence of life on Earth. In recent years, numerous important advances have been made towards understanding the influence that phosphates may have had on prebiotic chemistry, and here, we highlight two important aspects of prebiotic phosphate chemistry. Firstly, we discuss prebiotic...

1. A ribonuclease has been partially purified from the cotyledons of germinating seed of Pisum arvense. 2. The enzyme degrades ribopolynucleotides to adenosine 3'-phosphate, guanosine 3'-phosphate and the cyclic nucleotides cytidine 2',3'-phosphate and uridine 2',3'-phosphate; no resistant ;core' remains. 3. The activity of RNA-degrading enzymes in the cotyledons increases to a maximum during t...

The inorganic part of hard tissues (bones and teeth) of mammals consists of calcium phosphate, mainly of apatitic structure. Similarly, most undesired calcifications (i.e. those appearing as a result of various diseases) of mammals also contain calcium phosphate. For example, atherosclerosis results in blood-vessel blockage caused by a solid composite of cholesterol with calcium phosphate. Dent...