In a preliminary study, foam cells from atherosclerotic lesions have been isolated by incubation of the intima with collagenase and elastase. The composition and metabolism of homogeneous preparations of foam cells obtained in this way have been compared with other parts of the atherosclerotic intima. About 1% of the cholesterol and phospholipid of the intima was present in the cells obtained. ...

oxidation via the tricarboxylic acid cycle. The metabolism of [14C]ethanolamine to fatty acids via acetate has been shown to occur in mammals (Sprinson & Coulon, 1954; Sprinson & Weliky, 1969), but not previously ina bacterium. Thedecreased incorporation of [14C]ethanolamine that occurs when an alternative N source is present is probably related to the repression by (NH4)2S04 of the phospholyas...

The methylation steps in the biosynthesis of phosphatidylcholine by castor bean (Ricinus communis L.) endosperm have been studied by pulse-chase labeling. Endosperm halves were incubated with [methyl-(14)C]S-adenosyl-l-methionine, [2-(14)C]ethanolamine, [(14)C]ethanolamine phosphate, or [(14)C]serine phosphate. The kinetics of appearance were followed in the free, phospho-, and phosphatidyl-bas...

Hyaline membrane disease (HMD) is a disorder primarily affecting premature infants and is a significant cause of morbidity and mortality in this population. The disorder is characterized by widespread atelectasis and an inability of the neonate to expand his or her lungs. Secondary hemor rhage, edema and desquamation of alveolar lining cells then occur which further compromise respira tory func...

Euglena gracilis was grown in the dark for 12 days in a medium containing sodium acetate1-14C. The major amount of 14C was incorporated into the neutral lipids. In these compounds myristic acid was the main fatty acid labeled. Subsequent growth in light (70 hours) in an iden­ tical medium (containing unlabeled acetate) led to a strong decrease of radiocarbon in the neutral lipids. The radioacti...

The chemical composition of the lipophylic fraction from the halophytic plant Euphorbia peplis L. was investigated. Compared to other terrestrial higher plants an increase of triacylglycerols and especially of glycolipids was observed. The main phospholipid was phosphatidyl choline, followed by almost equal concentrations of phosphatidyl ethanolamine and phosphatidyl glycerol. A relatively high...

Essential-fatty acid deficiency produces a 52% increase in the rate of phosphatidyl-ethanolamine synthesis in rat liver as calculated from results obtained in vivo [Trewhella & Collins (1973) Biochem. Biophys. Acta 296, 34--50]. This flux change was used to test the possible regulatory roles of ethanolamine kinase and of phosphoethanolamine cytidylyltransferase, which are rate-limiting enzymes ...

The lipids extracted from rice brans were classified by thin-layer chromatography into eight fractions, and their fatty acid (FA) compositions were investigated among five different Japanese cultivars. The lipids of these rice brans comprised mainly triacylglycerols (TAG; 84.9-86.0 wt%), free FA (4.2-4.6 wt%), and phospholipids (PL; 6.5-6.7 wt%), whilst other components were also detected in mi...