Monoclonal antibodies (CGS-1, 2, 3, and 4) and rabbit antisera have been produced against bovine heart cyclic GMP-stimulated cyclic nucleotide phosphodiesterase. The monoclonal antibodies were all of the IgG1 subclass and had relatively high affinities (kd values, 1-5 X 10(-10) M), making them suitable for many sensitive immunological and analytical procedures. Most of the monoclonal antibodies...

Rehabilitation-dependent motor recovery after cerebral ischemia is associated with functional reorganization of residual cortical tissue. Recovery is thought to occur when remaining circuitry surrounding the lesion is "retrained" to assume some of the lost function. This reorganization is in turn supported by synaptic plasticity within cortical circuitry and manipulations that promote plasticit...

introduction: it was shown in a previous study, that mcpip, a cilostamide derivative, increased atrial contraction force without changing contraction rate. to improve this property, two new derivatives of cilostamide were synthesized and their effects on pde3 activity and isolated rat atria contraction were investigated. methods: the inhibitory effect of each compound on pde3 enzyme was determi...

In fragments of nucleic acids bearing 3’-monophosphoryl groups, both terminals may be determined by degrading the fragment with a massive dose of a single enzyme, venom phosphodiesterase (l-5). To be reliable, this method requires a preparation of phosphodiesterase which is both highly potent and free from contaminating interfering enzymes. The most dangerous contaminant is venom endonuclease, ...

Alzheimer’s disease is a memory-related neurodegenerative condition leading to cognitive impairment. Cholinergic deficit, together with other underlying mechanisms, leads the onset and progression of disease. Consequently, acetylcholinesterase inhibitors are used for symptomatic treatment dementia, even if limited efficacy observed. More recently, some specific phosphodiesterase isoforms emerge...

Cyclic nucleotide phosphodiesterases (PDEs) hydrolyze cAMP or cGMP and terminate their signaling. Two important families of PDEs that regulate cAMP signaling in cardiovascular tissues are the cGMP-inhibited PDEs (PDE3) and the cAMP-specific PDEs (PDE4). In this study, we have used a combination of an in vitro motility assay and a sensitive method for the measurement of cAMP in order to determin...

The calcium-dependent regulatory protein (CDR) purified from bovine brain was iodinated with Na[125I]I using the lactoperoxidase-glucose oxidase system. The iodinated protein retained its ability to stimulate the Ca2+-sensitive CDR-depleted cyclic nucleotide phosphodiesterase from bovine heart. Stimulation of the phosphodiesterase by 125I-CDR was Ca2+-dependent and the labeled protein had a Ka ...

A pheochromocytoma was maintained in rats from the New England Deaconess Hospital by giving the rats s.c. injections of isolated tumor cells. The animals were sac rificed 3 to 4 weeks after transplantation, the tumors were excised, and purified tumor cells were prepared. Cyclic nucleotide phosphodiesterase of the purified tumor cells was characterized and compared with that of the adrenal medul...